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@ARTICLE{Hilf:142870,
author = {N. Hilf and S. Kuttruff-Coqui and K. Frenzel and V. Bukur
and S. Stevanović$^*$ and C. Gouttefangeas$^*$ and M.
Platten$^*$ and G. Tabatabai$^*$ and V. Dutoit and S. H. van
der Burg and P. Thor Straten and F. Martínez-Ricarte and B.
Ponsati and H. Okada and U. Lassen and A. Admon and C. H.
Ottensmeier and A. Ulges and S. Kreiter and A. von
Deimling$^*$ and M. Skardelly and D. Migliorini and J. R.
Kroep and M. Idorn and J. Rodon and J. Piró and H. S.
Poulsen and B. Shraibman and K. McCann and R. Mendrzyk and
M. Löwer and M. Stieglbauer and C. M. Britten and D.
Capper$^*$ and M. J. P. Welters and J. Sahuquillo and K.
Kiesel and E. Derhovanessian and E. Rusch and L. Bunse$^*$
and C. Song and S. Heesch and C. Wagner and A. Kemmer-Brück
and J. Ludwig and J. C. Castle and O. Schoor and A. D.
Tadmor and E. Green$^*$ and J. Fritsche and M. Meyer and N.
Pawlowski and S. Dorner and F. Hoffgaard and B. Rössler and
D. Maurer and T. Weinschenk and C. Reinhardt and C. Huber
and H.-G. Rammensee$^*$ and H. Singh-Jasuja and U. Sahin and
P.-Y. Dietrich and W. Wick$^*$},
title = {{A}ctively personalized vaccination trial for newly
diagnosed glioblastoma.},
journal = {Nature},
volume = {565},
number = {7738},
issn = {1476-4687},
address = {London [u.a.]},
publisher = {Nature Publ. Group52462},
reportid = {DKFZ-2019-00500},
pages = {240 - 245},
year = {2019},
abstract = {Patients with glioblastoma currently do not sufficiently
benefit from recent breakthroughs in cancer treatment that
use checkpoint inhibitors1,2. For treatments using
checkpoint inhibitors to be successful, a high mutational
load and responses to neoepitopes are thought to be
essential3. There is limited intratumoural infiltration of
immune cells4 in glioblastoma and these tumours contain
only 30-50 non-synonymous mutations5. Exploitation of the
full repertoire of tumour antigens-that is, both unmutated
antigens and neoepitopes-may offer more effective
immunotherapies, especially for tumours with a low
mutational load. Here, in the phase I trial GAPVAC-101 of
the Glioma Actively Personalized Vaccine Consortium
(GAPVAC), we integrated highly individualized vaccinations
with both types of tumour antigens into standard care to
optimally exploit the limited target space for patients with
newly diagnosed glioblastoma. Fifteen patients with
glioblastomas positive for human leukocyte antigen
(HLA)-A*02:01 or HLA-A*24:02 were treated with a vaccine
(APVAC1) derived from a premanufactured library of unmutated
antigens followed by treatment with APVAC2, which
preferentially targeted neoepitopes. Personalization was
based on mutations and analyses of the transcriptomes and
immunopeptidomes of the individual tumours. The GAPVAC
approach was feasible and vaccines that had poly-ICLC
(polyriboinosinic-polyribocytidylic acid-poly-L-lysine
carboxymethylcellulose) and granulocyte-macrophage
colony-stimulating factor as adjuvants displayed favourable
safety and strong immunogenicity. Unmutated APVAC1 antigens
elicited sustained responses of central memory CD8+ T cells.
APVAC2 induced predominantly CD4+ T cell responses of T
helper 1 type against predicted neoepitopes.},
cin = {L801 / D170 / B300 / B320 / L101 / L501},
ddc = {500},
cid = {I:(DE-He78)L801-20160331 / I:(DE-He78)D170-20160331 /
I:(DE-He78)B300-20160331 / I:(DE-He78)B320-20160331 /
I:(DE-He78)L101-20160331 / I:(DE-He78)L501-20160331},
pnm = {312 - Functional and structural genomics (POF3-312)},
pid = {G:(DE-HGF)POF3-312},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:30568303},
doi = {10.1038/s41586-018-0810-y},
url = {https://inrepo02.dkfz.de/record/142870},
}