000147460 001__ 147460
000147460 005__ 20240229123024.0
000147460 0247_ $$2doi$$a10.1002/mp.13897
000147460 0247_ $$2pmid$$apmid:31677156
000147460 0247_ $$2ISSN$$a0094-2405
000147460 0247_ $$2ISSN$$a1522-8541
000147460 0247_ $$2ISSN$$a2473-4209
000147460 0247_ $$2altmetric$$aaltmetric:73291043
000147460 037__ $$aDKFZ-2019-02542
000147460 041__ $$aeng
000147460 082__ $$a610
000147460 1001_ $$aMcFadden, Conor H$$b0
000147460 245__ $$aIsolation of time dependent DNA damage induced by energetic carbon ions and their fragments using fluorescent nuclear track detectors.
000147460 260__ $$aCollege Park, Md.$$bAAPM$$c2020
000147460 3367_ $$2DRIVER$$aarticle
000147460 3367_ $$2DataCite$$aOutput Types/Journal article
000147460 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1583158000_30520
000147460 3367_ $$2BibTeX$$aARTICLE
000147460 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000147460 3367_ $$00$$2EndNote$$aJournal Article
000147460 500__ $$a 2020 Jan;47(1):272-281.
000147460 520__ $$aHigh energetic carbon (C-) ion beams undergo nuclear interactions with tissue producing secondary nuclear fragments. Thus, at depth, C-ion beams are composed of a mixture of different particles with different linear energy transfer (LET) values. We developed a technique to enable isolation of DNA damage response (DDR) in mixed radiation fields using beam line microscopy coupled to fluorescence nuclear track detectors (FNTDs).We imaged live cells on a coverslip made of FNTDs right after C-ion, proton or photon irradiation using an in-house built confocal microscope placed in the beam path. We used the FNTD to link track traversals with DNA damage and separated DNA damage induced by primary particles from fragments.We were able to spatially link physical parameters of radiation tracks to DDR in live cells to investigate spatiotemporal DDR in multi-ion radiation fields in real time, which was previously not possible. We demonstrated that the response of lesions produced by the high-LET primary particles associates most strongly with cell death in a multi-LET radiation field, and that this association is not seen when analyzing radiation induced foci in aggregate without primary/fragment classification.We report a new method that uses confocal microscopy in combination with FNTDs to provide sub-micrometer spatial-resolution measurements of radiation tracks in live cells. Our method facilitates expansion of the radiation-induced DDR research because it can be used in any particle beam line including particle therapy beam lines.
000147460 536__ $$0G:(DE-HGF)POF3-315$$a315 - Imaging and radiooncology (POF3-315)$$cPOF3-315$$fPOF III$$x0
000147460 588__ $$aDataset connected to CrossRef, PubMed,
000147460 7001_ $$0P:(DE-He78)fcbfc24123024baa698c6a693d60ab8a$$aRahmanian, Shirin$$b1$$udkfz
000147460 7001_ $$aFlint, David B$$b2
000147460 7001_ $$aBright, Scott J$$b3
000147460 7001_ $$aYoon, David S$$b4
000147460 7001_ $$aO'Brien, Daniel J$$b5
000147460 7001_ $$aAsaithamby, Aroumougame$$b6
000147460 7001_ $$0P:(DE-He78)360c5bc2b71a849e35aca747c041dda7$$aAbdollahi, Amir$$b7$$udkfz
000147460 7001_ $$0P:(DE-He78)bf44d68f90110cc79436dbb10f477518$$aGreilich, Steffen$$b8$$udkfz
000147460 7001_ $$aSawakuchi, Gabriel O$$b9
000147460 773__ $$0PERI:(DE-600)1466421-5$$a10.1002/mp.13897$$gp. mp.13897$$n1$$p272-281$$tMedical physics$$v47$$x2473-4209$$y2020
000147460 909CO $$ooai:inrepo02.dkfz.de:147460$$pVDB
000147460 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)fcbfc24123024baa698c6a693d60ab8a$$aDeutsches Krebsforschungszentrum$$b1$$kDKFZ
000147460 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)360c5bc2b71a849e35aca747c041dda7$$aDeutsches Krebsforschungszentrum$$b7$$kDKFZ
000147460 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)bf44d68f90110cc79436dbb10f477518$$aDeutsches Krebsforschungszentrum$$b8$$kDKFZ
000147460 9131_ $$0G:(DE-HGF)POF3-315$$1G:(DE-HGF)POF3-310$$2G:(DE-HGF)POF3-300$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lKrebsforschung$$vImaging and radiooncology$$x0
000147460 9141_ $$y2020
000147460 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline
000147460 915__ $$0StatID:(DE-HGF)0320$$2StatID$$aDBCoverage$$bPubMed Central
000147460 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bMED PHYS : 2017
000147460 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS
000147460 915__ $$0StatID:(DE-HGF)0600$$2StatID$$aDBCoverage$$bEbsco Academic Search
000147460 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bASC
000147460 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List
000147460 915__ $$0StatID:(DE-HGF)0110$$2StatID$$aWoS$$bScience Citation Index
000147460 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection
000147460 915__ $$0StatID:(DE-HGF)0111$$2StatID$$aWoS$$bScience Citation Index Expanded
000147460 915__ $$0StatID:(DE-HGF)1110$$2StatID$$aDBCoverage$$bCurrent Contents - Clinical Medicine
000147460 915__ $$0StatID:(DE-HGF)1030$$2StatID$$aDBCoverage$$bCurrent Contents - Life Sciences
000147460 915__ $$0StatID:(DE-HGF)9900$$2StatID$$aIF < 5
000147460 9201_ $$0I:(DE-He78)E040-20160331$$kE040$$lE040 Med. Physik in der Strahlentherapie$$x0
000147460 9201_ $$0I:(DE-He78)E210-20160331$$kE210$$lTranslationale Radioonkologie$$x1
000147460 9201_ $$0I:(DE-He78)HD01-20160331$$kHD01$$lDKTK HD zentral$$x2
000147460 980__ $$ajournal
000147460 980__ $$aVDB
000147460 980__ $$aI:(DE-He78)E040-20160331
000147460 980__ $$aI:(DE-He78)E210-20160331
000147460 980__ $$aI:(DE-He78)HD01-20160331
000147460 980__ $$aUNRESTRICTED