Journal Article

DKFZ-2019-02542

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Isolation of time dependent DNA damage induced by energetic carbon ions and their fragments using fluorescent nuclear track detectors.

McFadden, C. H. ; Rahmanian, S.DKFZ* ; Flint, D. B. ; Bright, S. J. ; Yoon, D. S. ; O'Brien, D. J. ; Asaithamby, A. ; Abdollahi, A.DKFZ* ; Greilich, S.DKFZ* ; Sawakuchi, G. O.

2020
AAPM College Park, Md.

This record in other databases:  

Please use a persistent id in citations: doi:10.1002/mp.13897

Abstract: High energetic carbon (C-) ion beams undergo nuclear interactions with tissue producing secondary nuclear fragments. Thus, at depth, C-ion beams are composed of a mixture of different particles with different linear energy transfer (LET) values. We developed a technique to enable isolation of DNA damage response (DDR) in mixed radiation fields using beam line microscopy coupled to fluorescence nuclear track detectors (FNTDs).We imaged live cells on a coverslip made of FNTDs right after C-ion, proton or photon irradiation using an in-house built confocal microscope placed in the beam path. We used the FNTD to link track traversals with DNA damage and separated DNA damage induced by primary particles from fragments.We were able to spatially link physical parameters of radiation tracks to DDR in live cells to investigate spatiotemporal DDR in multi-ion radiation fields in real time, which was previously not possible. We demonstrated that the response of lesions produced by the high-LET primary particles associates most strongly with cell death in a multi-LET radiation field, and that this association is not seen when analyzing radiation induced foci in aggregate without primary/fragment classification.We report a new method that uses confocal microscopy in combination with FNTDs to provide sub-micrometer spatial-resolution measurements of radiation tracks in live cells. Our method facilitates expansion of the radiation-induced DDR research because it can be used in any particle beam line including particle therapy beam lines.

Note: 2020 Jan;47(1):272-281.

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Contributing Institute(s):

1. E040 Med. Physik in der Strahlentherapie (E040)
2. Translationale Radioonkologie (E210)
3. DKTK HD zentral (HD01)

Research Program(s):

1. 315 - Imaging and radiooncology (POF3-315) (POF3-315)

Appears in the scientific report 2020

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Database coverage:
; Clarivate Analytics Master Journal List ; Current Contents - Clinical Medicine ; Current Contents - Life Sciences ; Ebsco Academic Search ; IF < 5 ; JCR ; PubMed Central ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Web of Science Core Collection

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