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@ARTICLE{McFadden:147460,
      author       = {C. H. McFadden and S. Rahmanian$^*$ and D. B. Flint and S.
                      J. Bright and D. S. Yoon and D. J. O'Brien and A. Asaithamby
                      and A. Abdollahi$^*$ and S. Greilich$^*$ and G. O.
                      Sawakuchi},
      title        = {{I}solation of time dependent {DNA} damage induced by
                      energetic carbon ions and their fragments using fluorescent
                      nuclear track detectors.},
      journal      = {Medical physics},
      volume       = {47},
      number       = {1},
      issn         = {2473-4209},
      address      = {College Park, Md.},
      publisher    = {AAPM},
      reportid     = {DKFZ-2019-02542},
      pages        = {272-281},
      year         = {2020},
      note         = {2020 Jan;47(1):272-281.},
      abstract     = {High energetic carbon (C-) ion beams undergo nuclear
                      interactions with tissue producing secondary nuclear
                      fragments. Thus, at depth, C-ion beams are composed of a
                      mixture of different particles with different linear energy
                      transfer (LET) values. We developed a technique to enable
                      isolation of DNA damage response (DDR) in mixed radiation
                      fields using beam line microscopy coupled to fluorescence
                      nuclear track detectors (FNTDs).We imaged live cells on a
                      coverslip made of FNTDs right after C-ion, proton or photon
                      irradiation using an in-house built confocal microscope
                      placed in the beam path. We used the FNTD to link track
                      traversals with DNA damage and separated DNA damage induced
                      by primary particles from fragments.We were able to
                      spatially link physical parameters of radiation tracks to
                      DDR in live cells to investigate spatiotemporal DDR in
                      multi-ion radiation fields in real time, which was
                      previously not possible. We demonstrated that the response
                      of lesions produced by the high-LET primary particles
                      associates most strongly with cell death in a multi-LET
                      radiation field, and that this association is not seen when
                      analyzing radiation induced foci in aggregate without
                      primary/fragment classification.We report a new method that
                      uses confocal microscopy in combination with FNTDs to
                      provide sub-micrometer spatial-resolution measurements of
                      radiation tracks in live cells. Our method facilitates
                      expansion of the radiation-induced DDR research because it
                      can be used in any particle beam line including particle
                      therapy beam lines.},
      cin          = {E040 / E210 / HD01},
      ddc          = {610},
      cid          = {I:(DE-He78)E040-20160331 / I:(DE-He78)E210-20160331 /
                      I:(DE-He78)HD01-20160331},
      pnm          = {315 - Imaging and radiooncology (POF3-315)},
      pid          = {G:(DE-HGF)POF3-315},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:31677156},
      doi          = {10.1002/mp.13897},
      url          = {https://inrepo02.dkfz.de/record/147460},
}