Journal Article

DKFZ-2020-00032

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Human mesenchymal stem cells are resistant to UV-B irradiation.

Lopez Perez, R. (First author)DKFZ* ; Brauer, J.DKFZ* ; Rühle, A.DKFZ* ; Trinh, T.DKFZ* ; Sisombath, S.DKFZ* ; Wuchter, P. ; Grosu, A.-L. ; Debus, J.DKFZ* ; Saffrich, R. ; Huber, P. E.DKFZ* ; Nicolay, N. (Last author)DKFZ*

2019
Macmillan Publishers Limited, part of Springer Nature [London]

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Please use a persistent id in citations: doi:10.1038/s41598-019-56591-9

Abstract: Albeit being an effective therapy for various cutaneous conditions, UV-B irradiation can cause severe skin damage. While multipotent mesenchymal stem cells (MSCs) may aid the regeneration of UV-B-induced skin injuries, the influence of UV-B irradiation on MSCs remains widely unknown. Here, we show that human MSCs are relatively resistant to UV-B irradiation compared to dermal fibroblasts. MSCs exhibited higher clonogenic survival, proliferative activity and viability than dermal fibroblasts after exposure to UV-B irradiation. Cellular adhesion, morphology and expression of characteristic surface marker patterns remained largely unaffected in UV-irradiated MSCs. The differentiation ability along the adipogenic, osteogenic and chondrogenic lineages was preserved after UV-B treatment. However, UV-B radiation resulted in a reduced ability of MSCs and dermal fibroblasts to migrate. MSCs exhibited low apoptosis rates after UV-B irradiation and repaired UV-B-induced cyclobutane pyrimidine dimers more efficiently than dermal fibroblasts. UV-B irradiation led to prolonged p53 protein stability and increased p21 protein expression resulting in a prolonged G2 arrest and senescence induction in MSCs. The observed resistance may contribute to the ability of these multipotent cells to aid the regeneration of UV-B-induced skin injuries.

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Contributing Institute(s):

1. Molekulare Radioonkologie (E055)

Research Program(s):

1. 315 - Imaging and radiooncology (POF3-315) (POF3-315)

Appears in the scientific report 2019

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