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@ARTICLE{Calandrini:154088,
author = {C. Calandrini and F. Schutgens and R. Oka and T. Margaritis
and T. Candelli and L. Mathijsen and C. Ammerlaan and R. L.
van Ineveld and S. Derakhshan and S. de Haan and E. Dolman
and P. Lijnzaad and L. Custers and H. Begthel and H. H. D.
Kerstens and L. L. Visser and M. Rookmaaker and M. Verhaar
and G. A. M. Tytgat and P. Kemmeren and R. R. de Krijger and
R. Al-Saadi and K. Pritchard-Jones and M. Kool$^*$ and A. C.
Rios and M. M. van den Heuvel-Eibrink and J. J. Molenaar and
R. van Boxtel and F. C. P. Holstege and H. Clevers and J.
Drost},
title = {{A}n organoid biobank for childhood kidney cancers that
captures disease and tissue heterogeneity.},
journal = {Nature Communications},
volume = {11},
number = {1},
issn = {2041-1723},
address = {[London]},
publisher = {Nature Publishing Group UK},
reportid = {DKFZ-2020-00554},
pages = {1310},
year = {2020},
abstract = {Kidney tumours are among the most common solid tumours in
children, comprising distinct subtypes differing in many
aspects, including cell-of-origin, genetics, and pathology.
Pre-clinical cell models capturing the disease heterogeneity
are currently lacking. Here, we describe the first
paediatric cancer organoid biobank. It contains tumour and
matching normal kidney organoids from over 50 children with
different subtypes of kidney cancer, including Wilms
tumours, malignant rhabdoid tumours, renal cell carcinomas,
and congenital mesoblastic nephromas. Paediatric kidney
tumour organoids retain key properties of native tumours,
useful for revealing patient-specific drug sensitivities.
Using single cell RNA-sequencing and high resolution 3D
imaging, we further demonstrate that organoid cultures
derived from Wilms tumours consist of multiple different
cell types, including epithelial, stromal and blastemal-like
cells. Our organoid biobank captures the heterogeneity of
paediatric kidney tumours, providing a representative
collection of well-characterised models for basic cancer
research, drug-screening and personalised medicine.},
cin = {B062 / HD01},
ddc = {500},
cid = {I:(DE-He78)B062-20160331 / I:(DE-He78)HD01-20160331},
pnm = {312 - Functional and structural genomics (POF3-312)},
pid = {G:(DE-HGF)POF3-312},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:32161258},
doi = {10.1038/s41467-020-15155-6},
url = {https://inrepo02.dkfz.de/record/154088},
}