Journal Article

DKFZ-2020-00554

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png An organoid biobank for childhood kidney cancers that captures disease and tissue heterogeneity.

Calandrini, C. ; Schutgens, F. ; Oka, R. ; Margaritis, T. ; Candelli, T. ; Mathijsen, L. ; Ammerlaan, C. ; van Ineveld, R. L. ; Derakhshan, S. ; de Haan, S. ; Dolman, E. ; Lijnzaad, P. ; Custers, L. ; Begthel, H. ; Kerstens, H. H. D. ; Visser, L. L. ; Rookmaaker, M. ; Verhaar, M. ; Tytgat, G. A. M. ; Kemmeren, P. ; de Krijger, R. R. ; Al-Saadi, R. ; Pritchard-Jones, K. ; Kool, M.DKFZ* ; Rios, A. C. ; van den Heuvel-Eibrink, M. M. ; Molenaar, J. J. ; van Boxtel, R. ; Holstege, F. C. P. ; Clevers, H. ; Drost, J.

2020
Nature Publishing Group UK [London]

This record in other databases:  

Please use a persistent id in citations: doi:10.1038/s41467-020-15155-6

Abstract: Kidney tumours are among the most common solid tumours in children, comprising distinct subtypes differing in many aspects, including cell-of-origin, genetics, and pathology. Pre-clinical cell models capturing the disease heterogeneity are currently lacking. Here, we describe the first paediatric cancer organoid biobank. It contains tumour and matching normal kidney organoids from over 50 children with different subtypes of kidney cancer, including Wilms tumours, malignant rhabdoid tumours, renal cell carcinomas, and congenital mesoblastic nephromas. Paediatric kidney tumour organoids retain key properties of native tumours, useful for revealing patient-specific drug sensitivities. Using single cell RNA-sequencing and high resolution 3D imaging, we further demonstrate that organoid cultures derived from Wilms tumours consist of multiple different cell types, including epithelial, stromal and blastemal-like cells. Our organoid biobank captures the heterogeneity of paediatric kidney tumours, providing a representative collection of well-characterised models for basic cancer research, drug-screening and personalised medicine.

---

Contributing Institute(s):

1. B062 Pädiatrische Neuroonkologie (B062)
2. DKTK HD zentral (HD01)

Research Program(s):

1. 312 - Functional and structural genomics (POF3-312) (POF3-312)

Appears in the scientific report 2020

---

Database coverage:
; ; ; BIOSIS Previews ; Clarivate Analytics Master Journal List ; Current Contents - Agriculture, Biology and Environmental Sciences ; Current Contents - Life Sciences ; Current Contents - Physical, Chemical and Earth Sciences ; DOAJ Seal ; IF >= 10 ; JCR ; NCBI Molecular Biology Database ; PubMed Central ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Web of Science Core Collection ; Zoological Record

---