Journal Article

DKFZ-2020-01024

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Process for an efficient lentiviral cell transduction.

Pirona, A. C. (First author)DKFZ* ; Oktriani, R.DKFZ* ; Boettcher, M. ; Hoheisel, J. (Last author)DKFZ*

2020
Oxford University Press Oxford

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Please use a persistent id in citations: doi:10.1093/biomethods/bpaa005

Abstract: The combination of lentiviruses with techniques such as CRISPR-Cas9 has resulted in efficient and precise processes for targeted genome modification. An often-limiting aspect, however, is the efficiency of cell transduction. Low efficiencies with particular cell types and/or the high complexity of lentiviral libraries can cause insufficient representation. Here, we present a protocol that yielded substantial increases in transduction efficiency in various cell lines in comparison to several other procedures.

Note: #EA:B070#LA:B070# / #DKFZ-MOST-Ca173#

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Contributing Institute(s):

1. AG Funktionelle Genomanalyse (B070)

Research Program(s):

1. 312 - Functional and structural genomics (POF3-312) (POF3-312)

Appears in the scientific report 2020

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