TY  - JOUR
AU  - Gojo, Johannes
AU  - Englinger, Bernhard
AU  - Jiang, Li
AU  - Hübner, Jens M
AU  - Shaw, McKenzie L
AU  - Hack, Olivia A
AU  - Madlener, Sibylle
AU  - Kirchhofer, Dominik
AU  - Liu, Ilon
AU  - Pyrdol, Jason
AU  - Hovestadt, Volker
AU  - Mazzola, Emanuele
AU  - Mathewson, Nathan D
AU  - Trissal, Maria
AU  - Lötsch, Daniela
AU  - Dorfer, Christian
AU  - Haberler, Christine
AU  - Halfmann, Angela
AU  - Mayr, Lisa
AU  - Peyrl, Andreas
AU  - Geyeregger, Rene
AU  - Schwalm, Benjamin
AU  - Mauermann, Monica
AU  - Pajtler, Kristian W
AU  - Milde, Till
AU  - Shore, Marni E
AU  - Geduldig, Jack E
AU  - Pelton, Kristine
AU  - Czech, Thomas
AU  - Ashenberg, Orr
AU  - Wucherpfennig, Kai W
AU  - Rozenblatt-Rosen, Orit
AU  - Alexandrescu, Sanda
AU  - Ligon, Keith L
AU  - Pfister, Stefan M
AU  - Regev, Aviv
AU  - Slavc, Irene
AU  - Berger, Walter
AU  - Suvà, Mario L
AU  - Kool, Marcel
AU  - Filbin, Mariella G
TI  - Single-Cell RNA-Seq Reveals Cellular Hierarchies and Impaired Developmental Trajectories in Pediatric Ependymoma.
JO  - Cancer cell
VL  - 38
IS  - 1
SN  - 1535-6108
CY  - New York, NY
PB  - Elsevier
M1  - DKFZ-2020-01427
SP  - 44 - 59.e9
PY  - 2020
N1  - #LA:B062#
AB  - Ependymoma is a heterogeneous entity of central nervous system tumors with well-established molecular groups. Here, we apply single-cell RNA sequencing to analyze ependymomas across molecular groups and anatomic locations to investigate their intratumoral heterogeneity and developmental origins. Ependymomas are composed of a cellular hierarchy initiating from undifferentiated populations, which undergo impaired differentiation toward three lineages of neuronal-glial fate specification. While prognostically favorable groups of ependymoma predominantly harbor differentiated cells, aggressive groups are enriched for undifferentiated cell populations. The delineated transcriptomic signatures correlate with patient survival and define molecular dependencies for targeted treatment approaches. Taken together, our analyses reveal a developmental hierarchy underlying ependymomas relevant to biological and clinical behavior.
LB  - PUB:(DE-HGF)16
C6  - pmid:32663469
DO  - DOI:10.1016/j.ccell.2020.06.004
UR  - https://inrepo02.dkfz.de/record/157141
ER  -