Journal Article

DKFZ-2020-01427

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Single-Cell RNA-Seq Reveals Cellular Hierarchies and Impaired Developmental Trajectories in Pediatric Ependymoma.

Gojo, J. ; Englinger, B. ; Jiang, L. ; Hübner, J. M.DKFZ* ; Shaw, M. L. ; Hack, O. A. ; Madlener, S. ; Kirchhofer, D. ; Liu, I. ; Pyrdol, J. ; Hovestadt, V. ; Mazzola, E. ; Mathewson, N. D. ; Trissal, M. ; Lötsch, D. ; Dorfer, C. ; Haberler, C. ; Halfmann, A. ; Mayr, L. ; Peyrl, A. ; Geyeregger, R. ; Schwalm, B.DKFZ* ; Mauermann, M. ; Pajtler, K. W.DKFZ* ; Milde, T.DKFZ* ; Shore, M. E. ; Geduldig, J. E. ; Pelton, K. ; Czech, T. ; Ashenberg, O. ; Wucherpfennig, K. W. ; Rozenblatt-Rosen, O. ; Alexandrescu, S. ; Ligon, K. L. ; Pfister, S. M.DKFZ* ; Regev, A. ; Slavc, I. ; Berger, W. ; Suvà, M. L. ; Kool, M. (Last author)DKFZ* ; Filbin, M. G.

2020
Elsevier New York, NY

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Please use a persistent id in citations: doi:10.1016/j.ccell.2020.06.004

Abstract: Ependymoma is a heterogeneous entity of central nervous system tumors with well-established molecular groups. Here, we apply single-cell RNA sequencing to analyze ependymomas across molecular groups and anatomic locations to investigate their intratumoral heterogeneity and developmental origins. Ependymomas are composed of a cellular hierarchy initiating from undifferentiated populations, which undergo impaired differentiation toward three lineages of neuronal-glial fate specification. While prognostically favorable groups of ependymoma predominantly harbor differentiated cells, aggressive groups are enriched for undifferentiated cell populations. The delineated transcriptomic signatures correlate with patient survival and define molecular dependencies for targeted treatment approaches. Taken together, our analyses reveal a developmental hierarchy underlying ependymomas relevant to biological and clinical behavior.

Note: #LA:B062#

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Contributing Institute(s):

1. B062 Pädiatrische Neuroonkologie (B062)
2. KKE Pädiatrische Onkologie (B310)

Research Program(s):

1. 312 - Functional and structural genomics (POF3-312) (POF3-312)

Appears in the scientific report 2020

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Database coverage:
; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Clinical Medicine ; Current Contents - Life Sciences ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 20 ; JCR ; NCBI Molecular Biology Database ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Web of Science Core Collection

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