guest ::
| Home > Publications database > Carbon ion dosimetry on a fluorescent nuclear track detector using widefield microscopy. |
| Home > Publications database > Carbon ion dosimetry on a fluorescent nuclear track detector using widefield microscopy. |
| Journal Article | DKFZ-2020-01884 |
; ; ; ; ;
2020
IOP Publ.
Bristol
Abstract: Fluorescent nuclear track detectors (FNTD) are solid-state dosimeters used in a wide range of dosimetric and biomedical applications in research worldwide. FNTDs are a core but currently underutilized dosimetry tool in the field of radiation biology which are inherently capable of visualizing the tracks of ions used in hadron therapy. The ions that traverse the FNTD deposit their energy according to their linear energy transfer (LET) and transform colour centres to form trackspots around their trajectory. These trackspots are the fingerprints of the ions which have fluorescent properties (Ex:620 nm Em:750 nm). The trackspots in the FNTD can be visualized by fluorescence microscopy enabling a well-defined dosimetric readout with a spatial component indicating the trajectory of individual ions. The current method used to analyse the FNTDs is laser scanning confocal microscopy (LSM). LSM enables a precise localization of track spots in x, y and z however due to the scanning of the laser spot across the sample, requires a long time for large samples. This body of work conclusively shows for the first time that the readout of the trackspots present after irradiation (0.5 Gy carbon ions) in the FNTD can be captured with a widefield microscope (WF). This method was developed to optimize the readout of the Cell-FIT-HD biomedical sensor based on a Landauer Al2O3:C,Mg FNTD used for biological single cell dosimetry. The work in this paper shows the use of a widefield microscope to image the tracks in the FNTD. The WF readout of the FNTD is a factor ~10 faster, for an area 2.97 times the size making the method nearly a factor 19 faster in track acquisition than LSM. This dramatic increase in image acquisition will replace LSM in the Cell-FIT-HD workflow enabling a higher throughput of samples.
; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; Ebsco Academic Search ; Essential Science Indicators ; IF < 5 ; JCR ; National-Konsortium ; Nationallizenz
; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Web of Science Core Collection
|
The record appears in these collections: |
