Master Thesis

DKFZ-2020-02656

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Development of a Novel Method for Measuring theOxygen Consumption Rate of Cancer Cells in Vitrousing Quenched Luminescence Oxygen Sensors

Hehn, L.DKFZ*

2020

Abstract: The increase of cellular radiosensitivity due to the presence of oxygen has been widelyestablished [1]–[3]. Comparatively little is known about the effect of cellular oxygenconsumption on the results of radiotherapy, however.In this project, a novel method for measuring cellular oxygen consumption in vitro wasdeveloped and used to measure the oxygen consumption rate of eight cancer cell lines.The rates were compared with data from clonogenic assays of these cell lines to investigateto which extent the oxygen consumption rate can make predictions of cellularradiosensitivity.Oxygen sensing was done using non-invasive sensors based on the principle of quenchedluminescence, which allow accurate, real-time measurements that do not disturb thesample.

Note: Masterarbeit, Universität Heidelberg, 2020

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Contributing Institute(s):

1. E041 Medizinische Physik in der Radioonkologie (E041)

Research Program(s):

1. 315 - Imaging and radiooncology (POF3-315) (POF3-315)

Appears in the scientific report 2020

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