TY  - JOUR
AU  - Costa, Barbara Maria
AU  - Fletcher, Michael
AU  - Boskovic, Pavle
AU  - Ivanova, Ekaterina L
AU  - Eisemann, Tanja
AU  - Lohr, Sabrina
AU  - Bunse, Lukas
AU  - Löwer, Martin
AU  - Burchard, Stefanie
AU  - Korshunov, Andrey
AU  - Coltella, Nadia
AU  - Cusimano, Melania
AU  - Naldini, Luigi
AU  - Liu, Hai-Kun
AU  - Platten, Michael
AU  - Radlwimmer, Bernhard
AU  - Angel, Peter
AU  - Peterziel, Heike
TI  - A Set of Cell Lines Derived from a Genetic Murine Glioblastoma Model Recapitulates Molecular and Morphological Characteristics of Human Tumors.
JO  - Cancers
VL  - 13
IS  - 2
SN  - 2072-6694
CY  - Basel
PB  - MDPI
M1  - DKFZ-2021-00109
SP  - 230
PY  - 2021
N1  - DKFZ-ZMBH Alliance#EA:A100#LA:A100# / HI-TRON
AB  - Glioblastomas (GBM) are the most aggressive tumors affecting the central nervous system in adults, causing death within, on average, 15 months after diagnosis. Immunocompetent in-vivo models that closely mirror human GBM are urgently needed for deciphering glioma biology and for the development of effective treatment options. The murine GBM cell lines currently available for engraftment in immunocompetent mice are not only exiguous but also inadequate in representing prominent characteristics of human GBM such as infiltrative behavior, necrotic areas, and pronounced tumor heterogeneity. Therefore, we generated a set of glioblastoma cell lines by repeated in vivo passaging of cells isolated from a neural stem cell-specific Pten/p53 double-knockout genetic mouse brain tumor model. Transcriptome and genome analyses of the cell lines revealed molecular heterogeneity comparable to that observed in human glioblastoma. Upon orthotopic transplantation into syngeneic hosts, they formed high-grade gliomas that faithfully recapitulated the histopathological features, invasiveness and immune cell infiltration characteristic of human glioblastoma. These features make our cell lines unique and useful tools to study multiple aspects of glioblastoma pathomechanism and to test novel treatments in an intact immune microenvironment.
KW  - glioblastoma (Other)
KW  - mouse model (Other)
KW  - syngeneic cell line (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:33435218
DO  - DOI:10.3390/cancers13020230
UR  - https://inrepo02.dkfz.de/record/166770
ER  -