Journal Article

DKFZ-2021-00109

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png A Set of Cell Lines Derived from a Genetic Murine Glioblastoma Model Recapitulates Molecular and Morphological Characteristics of Human Tumors.

Costa, B. M. (First author)DKFZ* ; Fletcher, M.DKFZ* ; Boskovic, P.DKFZ* ; Ivanova, E. L.DKFZ* ; Eisemann, T.DKFZ* ; Lohr, S.DKFZ* ; Bunse, L.DKFZ* ; Löwer, M. ; Burchard, S. ; Korshunov, A.DKFZ* ; Coltella, N. ; Cusimano, M. ; Naldini, L. ; Liu, H.-K.DKFZ* ; Platten, M.DKFZ* ; Radlwimmer, B.DKFZ* ; Angel, P.DKFZ* ; Peterziel, H. (Last author)DKFZ*

2021
MDPI Basel

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Please use a persistent id in citations: doi:10.3390/cancers13020230

Abstract: Glioblastomas (GBM) are the most aggressive tumors affecting the central nervous system in adults, causing death within, on average, 15 months after diagnosis. Immunocompetent in-vivo models that closely mirror human GBM are urgently needed for deciphering glioma biology and for the development of effective treatment options. The murine GBM cell lines currently available for engraftment in immunocompetent mice are not only exiguous but also inadequate in representing prominent characteristics of human GBM such as infiltrative behavior, necrotic areas, and pronounced tumor heterogeneity. Therefore, we generated a set of glioblastoma cell lines by repeated in vivo passaging of cells isolated from a neural stem cell-specific Pten/p53 double-knockout genetic mouse brain tumor model. Transcriptome and genome analyses of the cell lines revealed molecular heterogeneity comparable to that observed in human glioblastoma. Upon orthotopic transplantation into syngeneic hosts, they formed high-grade gliomas that faithfully recapitulated the histopathological features, invasiveness and immune cell infiltration characteristic of human glioblastoma. These features make our cell lines unique and useful tools to study multiple aspects of glioblastoma pathomechanism and to test novel treatments in an intact immune microenvironment.

Keyword(s): glioblastoma ; mouse model ; syngeneic cell line

Note: DKFZ-ZMBH Alliance#EA:A100#LA:A100# / HI-TRON

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Contributing Institute(s):

1. A100 Signaltransduktion/ Wachstumskontrolle (A100)
2. B060 Molekulare Genetik (B060)
3. KKE Neuroimmunologie und Hirntumorimmunologie (D170)
4. KKE Neuropathologie (B300)
5. A240 Molekulare Neurogenetik (A240)
6. DKTK HD zentral (HD01)

Research Program(s):

1. 311 - Zellbiologie und Tumorbiologie (POF4-311) (POF4-311)

Appears in the scientific report 2021

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Database coverage:
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