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@ARTICLE{Luck:170294,
author = {R. Luck and A. Karakatsani and B. Shah and G. Schermann and
H. Adler and J. Kupke and N. Tisch and H.-W. Jeong and M. K.
Back and F. Hetsch and A. D'Errico and M. De Palma and E.
Wiedtke and D. Grimm and A. Acker-Palmer and J. von
Engelhardt and R. H. Adams and H. G. Augustin$^*$ and C.
Ruiz de Almodóvar},
title = {{T}he angiopoietin-{T}ie2 pathway regulates {P}urkinje cell
dendritic morphogenesis in a cell-autonomous manner.},
journal = {Cell reports},
volume = {36},
number = {7},
issn = {2211-1247},
address = {[New York, NY]},
publisher = {Elsevier},
reportid = {DKFZ-2021-01884},
pages = {109522},
year = {2021},
note = {DKFZ-ZMBH Alliance},
abstract = {Neuro-vascular communication is essential to synchronize
central nervous system development. Here, we identify
angiopoietin/Tie2 as a neuro-vascular signaling axis
involved in regulating dendritic morphogenesis of Purkinje
cells (PCs). We show that in the developing cerebellum Tie2
expression is not restricted to blood vessels, but it is
also present in PCs. Its ligands angiopoietin-1 (Ang1) and
angiopoietin-2 (Ang2) are expressed in neural cells and
endothelial cells (ECs), respectively. PC-specific deletion
of Tie2 results in reduced dendritic arborization, which is
recapitulated in neural-specific Ang1-knockout and Ang2
full-knockout mice. Mechanistically, RNA sequencing reveals
that Tie2-deficient PCs present alterations in gene
expression of multiple genes involved in cytoskeleton
organization, dendritic formation, growth, and branching.
Functionally, mice with deletion of Tie2 in PCs present
alterations in PC network functionality. Altogether, our
data propose Ang/Tie2 signaling as a mediator of
intercellular communication between neural cells, ECs, and
PCs, required for proper PC dendritic morphogenesis and
function.},
keywords = {Purkinje cell (Other) / Tie2 (Other) / angiogenesis (Other)
/ angipoietin (Other) / blood vessels (Other) / cerebellum
(Other) / dendritic branching (Other) / dendritic morphology
(Other) / neuro-vascular (Other) / neurodevelopment (Other)},
cin = {A190},
ddc = {610},
cid = {I:(DE-He78)A190-20160331},
pnm = {311 - Zellbiologie und Tumorbiologie (POF4-311)},
pid = {G:(DE-HGF)POF4-311},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:34407407},
doi = {10.1016/j.celrep.2021.109522},
url = {https://inrepo02.dkfz.de/record/170294},
}