Journal Article

DKFZ-2021-01884

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png The angiopoietin-Tie2 pathway regulates Purkinje cell dendritic morphogenesis in a cell-autonomous manner.

Luck, R. ; Karakatsani, A. ; Shah, B. ; Schermann, G. ; Adler, H. ; Kupke, J. ; Tisch, N. ; Jeong, H.-W. ; Back, M. K. ; Hetsch, F. ; D'Errico, A. ; De Palma, M. ; Wiedtke, E. ; Grimm, D. ; Acker-Palmer, A. ; von Engelhardt, J. ; Adams, R. H. ; Augustin, H. G.DKFZ* ; Ruiz de Almodóvar, C.

2021
Elsevier [New York, NY]

This record in other databases:  

Please use a persistent id in citations: doi:10.1016/j.celrep.2021.109522

Abstract: Neuro-vascular communication is essential to synchronize central nervous system development. Here, we identify angiopoietin/Tie2 as a neuro-vascular signaling axis involved in regulating dendritic morphogenesis of Purkinje cells (PCs). We show that in the developing cerebellum Tie2 expression is not restricted to blood vessels, but it is also present in PCs. Its ligands angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2) are expressed in neural cells and endothelial cells (ECs), respectively. PC-specific deletion of Tie2 results in reduced dendritic arborization, which is recapitulated in neural-specific Ang1-knockout and Ang2 full-knockout mice. Mechanistically, RNA sequencing reveals that Tie2-deficient PCs present alterations in gene expression of multiple genes involved in cytoskeleton organization, dendritic formation, growth, and branching. Functionally, mice with deletion of Tie2 in PCs present alterations in PC network functionality. Altogether, our data propose Ang/Tie2 signaling as a mediator of intercellular communication between neural cells, ECs, and PCs, required for proper PC dendritic morphogenesis and function.

Keyword(s): Purkinje cell ; Tie2 ; angiogenesis ; angipoietin ; blood vessels ; cerebellum ; dendritic branching ; dendritic morphology ; neuro-vascular ; neurodevelopment

Note: DKFZ-ZMBH Alliance

---

Contributing Institute(s):

1. A190 Vaskuläre Onkologie und Metastatistierung (A190)

Research Program(s):

1. 311 - Zellbiologie und Tumorbiologie (POF4-311) (POF4-311)

Appears in the scientific report 2021

---

Database coverage:
; ; ; Article Processing Charges ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; DOAJ Seal ; Essential Science Indicators ; Fees ; IF >= 5 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

---