TY  - JOUR
AU  - Sarropoulos, Ioannis
AU  - Sepp, Mari
AU  - Frömel, Robert
AU  - Leiss, Kevin
AU  - Trost, Nils
AU  - Leushkin, Evgeny
AU  - Okonechnikov, Konstantin
AU  - Joshi, Piyush Kumar
AU  - Giere, Peter
AU  - Kutscher, Lena
AU  - Cardoso-Moreira, Margarida
AU  - Pfister, Stefan
AU  - Kaessmann, Henrik
TI  - Developmental and evolutionary dynamics of cis-regulatory elements in mouse cerebellar cells.
JO  - Science
VL  - 373
IS  - 6558
SN  - 0036-8075
CY  - Cambridge, Mass.
PB  - Moses King
M1  - DKFZ-2021-01940
SP  - eabg4696 -
PY  - 2021
N1  - #LA:B062#
AB  - Organ development is orchestrated by cell- and time-specific gene regulatory networks. In this study, we investigated the regulatory basis of mouse cerebellum development from early neurogenesis to adulthood. By acquiring snATAC-seq (single-nucleus assay for transposase accessible chromatin using sequencing) profiles for  90,000 cells spanning 11 stages, we mapped cerebellar cell types and identified candidate cis-regulatory elements (CREs). We detected extensive spatiotemporal heterogeneity among progenitor cells and a gradual divergence in the regulatory programs of cerebellar neurons during differentiation. Comparisons to vertebrate genomes and snATAC-seq profiles for ∼20,000 cerebellar cells from the marsupial opossum revealed a shared decrease in CRE conservation during development and differentiation as well as differences in constraint between cell types. Our work delineates the developmental and evolutionary dynamics of gene regulation in cerebellar cells and provides insights into mammalian organ development.
LB  - PUB:(DE-HGF)16
C6  - pmid:34446581
DO  - DOI:10.1126/science.abg4696
UR  - https://inrepo02.dkfz.de/record/170447
ER  -