Journal Article

DKFZ-2021-01940

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Developmental and evolutionary dynamics of cis-regulatory elements in mouse cerebellar cells.

Sarropoulos, I. ; Sepp, M. ; Frömel, R. ; Leiss, K. ; Trost, N. ; Leushkin, E. ; Okonechnikov, K.DKFZ* ; Joshi, P. K.DKFZ* ; Giere, P. ; Kutscher, L.DKFZ* ; Cardoso-Moreira, M. ; Pfister, S. (Last author)DKFZ* ; Kaessmann, H.

2021
Moses King Cambridge, Mass.

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Please use a persistent id in citations: doi:10.1126/science.abg4696

Abstract: Organ development is orchestrated by cell- and time-specific gene regulatory networks. In this study, we investigated the regulatory basis of mouse cerebellum development from early neurogenesis to adulthood. By acquiring snATAC-seq (single-nucleus assay for transposase accessible chromatin using sequencing) profiles for ~90,000 cells spanning 11 stages, we mapped cerebellar cell types and identified candidate cis-regulatory elements (CREs). We detected extensive spatiotemporal heterogeneity among progenitor cells and a gradual divergence in the regulatory programs of cerebellar neurons during differentiation. Comparisons to vertebrate genomes and snATAC-seq profiles for ∼20,000 cerebellar cells from the marsupial opossum revealed a shared decrease in CRE conservation during development and differentiation as well as differences in constraint between cell types. Our work delineates the developmental and evolutionary dynamics of gene regulation in cerebellar cells and provides insights into mammalian organ development.

Note: #LA:B062#

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Contributing Institute(s):

1. B062 Pädiatrische Neuroonkologie (B062)
2. DKTK HD zentral (HD01)
3. Entwicklungsbiolog. Ursprünge pädriatischer Krebserkrankungen (B430)

Research Program(s):

1. 312 - Funktionelle und strukturelle Genomforschung (POF4-312) (POF4-312)

Appears in the scientific report 2021

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Database coverage:
; BIOSIS Previews ; Biological Abstracts ; Chemical Reactions ; Clarivate Analytics Master Journal List ; Current Contents - Agriculture, Biology and Environmental Sciences ; Current Contents - Life Sciences ; Current Contents - Physical, Chemical and Earth Sciences ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 40 ; Index Chemicus ; JCR ; National-Konsortium ; Nationallizenz ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection ; Zoological Record

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