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@ARTICLE{Sarropoulos:170447,
author = {I. Sarropoulos and M. Sepp and R. Frömel and K. Leiss and
N. Trost and E. Leushkin and K. Okonechnikov$^*$ and P. K.
Joshi$^*$ and P. Giere and L. Kutscher$^*$ and M.
Cardoso-Moreira and S. Pfister$^*$ and H. Kaessmann},
title = {{D}evelopmental and evolutionary dynamics of cis-regulatory
elements in mouse cerebellar cells.},
journal = {Science},
volume = {373},
number = {6558},
issn = {0036-8075},
address = {Cambridge, Mass.},
publisher = {Moses King},
reportid = {DKFZ-2021-01940},
pages = {eabg4696 -},
year = {2021},
note = {#LA:B062#},
abstract = {Organ development is orchestrated by cell- and
time-specific gene regulatory networks. In this study, we
investigated the regulatory basis of mouse cerebellum
development from early neurogenesis to adulthood. By
acquiring snATAC-seq (single-nucleus assay for transposase
accessible chromatin using sequencing) profiles for ~90,000
cells spanning 11 stages, we mapped cerebellar cell types
and identified candidate cis-regulatory elements (CREs). We
detected extensive spatiotemporal heterogeneity among
progenitor cells and a gradual divergence in the regulatory
programs of cerebellar neurons during differentiation.
Comparisons to vertebrate genomes and snATAC-seq profiles
for ∼20,000 cerebellar cells from the marsupial opossum
revealed a shared decrease in CRE conservation during
development and differentiation as well as differences in
constraint between cell types. Our work delineates the
developmental and evolutionary dynamics of gene regulation
in cerebellar cells and provides insights into mammalian
organ development.},
cin = {B062 / HD01 / B430},
ddc = {500},
cid = {I:(DE-He78)B062-20160331 / I:(DE-He78)HD01-20160331 /
I:(DE-He78)B430-20160331},
pnm = {312 - Funktionelle und strukturelle Genomforschung
(POF4-312)},
pid = {G:(DE-HGF)POF4-312},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:34446581},
doi = {10.1126/science.abg4696},
url = {https://inrepo02.dkfz.de/record/170447},
}