000177452 001__ 177452
000177452 005__ 20240229133740.0
000177452 037__ $$aDKFZ-2021-02544
000177452 1001_ $$0P:(DE-He78)4c81b9a69bd0a49e7ca831302934d68e$$aMilewski, Karolin$$b0
000177452 245__ $$aThe damage response of human cancercells to irradiation under the variation ofthe dose rate and oxygen level
000177452 260__ $$c2021
000177452 3367_ $$2DRIVER$$abachelorThesis
000177452 3367_ $$02$$2EndNote$$aThesis
000177452 3367_ $$2DataCite$$aOutput Types/Supervised Student Publication
000177452 3367_ $$0PUB:(DE-HGF)2$$2PUB:(DE-HGF)$$aBachelor Thesis$$bbachelor$$mbachelor$$s1637151530_23141
000177452 3367_ $$2BibTeX$$aMASTERSTHESIS
000177452 3367_ $$2ORCID$$aSUPERVISED_STUDENT_PUBLICATION
000177452 500__ $$aCorresponding author J. Seco
000177452 502__ $$aBachelorarbeit, Universität Heidelberg, 2021$$bBachelorarbeit$$cUniversität Heidelberg$$gFakultät für Physik und Astronomie
000177452 520__ $$aIn radiotherapy, it has always been the main objective to effectively treat tumours, while damaginghealthy tissue as little as possible. It has been found in several in vivo studies that irradiation athigh dose rates spares healthy tissue, while remaining the same tumour control as with conventional dose rates, allowing for healthy and cancerous tissue to self-delimit when irradiated withhigh dose rates. In vitro, a sparing effect after irradiation with high dose rates in comparison toconventional dose rates was observed even for cells of the same cell line. This sparing effect iscalled FLASH effect and has been found to be closely related to the oxygen concentration in thecells during the irradiation, but the underlying mechanism is still unclear.Hence this Bachelor’s thesis investigated the damage response of cells of the human pancreaticcancer cell line Panc-1 to the irradiation with X-rays at different dose rates under the influence ofdifferent oxygen concentrations in the cellular environment. Clonogenic cell survival assays havebeen carried out at four different oxygen levels up to 2 % in order to narrow down the oxygenconcentration window, in which the enhancement of radioresistance through high dose rates ispresent.The present experiments have, first of all, confirmed that less oxygenated cells are less radiosensitive. Furthermore, it has been found that the enhancement of the radioresistance of cells throughhigh dose rates diminishes with decreasing oxygen levels and, against all expectations, is strongestunder normoxic conditions. This observation has already been made by an other research group[2] and opens up many new question for further research.
000177452 536__ $$0G:(DE-HGF)POF4-315$$a315 - Bildgebung und Radioonkologie (POF4-315)$$cPOF4-315$$fPOF IV$$x0
000177452 909CO $$ooai:inrepo02.dkfz.de:177452$$pVDB
000177452 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)4c81b9a69bd0a49e7ca831302934d68e$$aDeutsches Krebsforschungszentrum$$b0$$kDKFZ
000177452 9131_ $$0G:(DE-HGF)POF4-315$$1G:(DE-HGF)POF4-310$$2G:(DE-HGF)POF4-300$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lKrebsforschung$$vBildgebung und Radioonkologie$$x0
000177452 9141_ $$y2021
000177452 9201_ $$0I:(DE-He78)E041-20160331$$kE041$$lE041 Medizinische Physik in der Radioonkologie$$x0
000177452 980__ $$abachelor
000177452 980__ $$aVDB
000177452 980__ $$aI:(DE-He78)E041-20160331
000177452 980__ $$aUNRESTRICTED