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| Home > Publications database > The damage response of human cancercells to irradiation under the variation ofthe dose rate and oxygen level |
| Bachelor Thesis | DKFZ-2021-02544 |
2021
Abstract: In radiotherapy, it has always been the main objective to effectively treat tumours, while damaginghealthy tissue as little as possible. It has been found in several in vivo studies that irradiation athigh dose rates spares healthy tissue, while remaining the same tumour control as with conventional dose rates, allowing for healthy and cancerous tissue to self-delimit when irradiated withhigh dose rates. In vitro, a sparing effect after irradiation with high dose rates in comparison toconventional dose rates was observed even for cells of the same cell line. This sparing effect iscalled FLASH effect and has been found to be closely related to the oxygen concentration in thecells during the irradiation, but the underlying mechanism is still unclear.Hence this Bachelor’s thesis investigated the damage response of cells of the human pancreaticcancer cell line Panc-1 to the irradiation with X-rays at different dose rates under the influence ofdifferent oxygen concentrations in the cellular environment. Clonogenic cell survival assays havebeen carried out at four different oxygen levels up to 2 % in order to narrow down the oxygenconcentration window, in which the enhancement of radioresistance through high dose rates ispresent.The present experiments have, first of all, confirmed that less oxygenated cells are less radiosensitive. Furthermore, it has been found that the enhancement of the radioresistance of cells throughhigh dose rates diminishes with decreasing oxygen levels and, against all expectations, is strongestunder normoxic conditions. This observation has already been made by an other research group[2] and opens up many new question for further research.
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