TY  - THES
AU  - Milewski, Karolin
TI  - The damage response of human cancercells to irradiation under the variation ofthe dose rate and oxygen level
PB  - Universität Heidelberg
VL  - Bachelorarbeit
M1  - DKFZ-2021-02544
PY  - 2021
N1  - Corresponding author J. Seco
N1  - Bachelorarbeit, Universität Heidelberg, 2021
AB  - In radiotherapy, it has always been the main objective to effectively treat tumours, while damaginghealthy tissue as little as possible. It has been found in several in vivo studies that irradiation athigh dose rates spares healthy tissue, while remaining the same tumour control as with conventional dose rates, allowing for healthy and cancerous tissue to self-delimit when irradiated withhigh dose rates. In vitro, a sparing effect after irradiation with high dose rates in comparison toconventional dose rates was observed even for cells of the same cell line. This sparing effect iscalled FLASH effect and has been found to be closely related to the oxygen concentration in thecells during the irradiation, but the underlying mechanism is still unclear.Hence this Bachelor’s thesis investigated the damage response of cells of the human pancreaticcancer cell line Panc-1 to the irradiation with X-rays at different dose rates under the influence ofdifferent oxygen concentrations in the cellular environment. Clonogenic cell survival assays havebeen carried out at four different oxygen levels up to 2 
LB  - PUB:(DE-HGF)2
UR  - https://inrepo02.dkfz.de/record/177452
ER  -