000177454 001__ 177454
000177454 005__ 20240229133741.0
000177454 037__ $$aDKFZ-2021-02546
000177454 1001_ $$0P:(DE-He78)0ba9f9aa0f67731222213b440c10ab5c$$aWüllner, Amelie$$b0
000177454 245__ $$aToxicity studies on lung and pancreatic cancer celllines: An approach towards FLASH mechanisms
000177454 260__ $$c2021
000177454 3367_ $$2DRIVER$$abachelorThesis
000177454 3367_ $$02$$2EndNote$$aThesis
000177454 3367_ $$2DataCite$$aOutput Types/Supervised Student Publication
000177454 3367_ $$0PUB:(DE-HGF)2$$2PUB:(DE-HGF)$$aBachelor Thesis$$bbachelor$$mbachelor$$s1637151351_19386
000177454 3367_ $$2BibTeX$$aMASTERSTHESIS
000177454 3367_ $$2ORCID$$aSUPERVISED_STUDENT_PUBLICATION
000177454 500__ $$aCorresponding author J. Seco
000177454 502__ $$aBachelorarbeit, Universität Heidelberg, 2021$$bBachelorarbeit$$cUniversität Heidelberg$$gFakultät für Physik und Astronomie
000177454 520__ $$aSeveral studies have hinted towards involvement of radical scavengers in the sparingof healthy tissue in radiotherapy (RT) for ultra-high dose rates (FLASH effect). Thesaturation of the intracellular enzyme Superoxide Dismutase (SOD) is proposed to playa key role in the FLASH effect. The aim of the current study was to establish treatmentprotocols to achieve 80% cell viability of two cancer cell lines for four compounds thataffect the enzyme and its substrate Superoxide. Cytotoxicity assays were done on theH460 lung cancer cell line and the Panc-1 pancreatic cancer cell line with the compoundsH2O2, MSB, NaCN and KCN. The effect of Catalase on cell viability was tested and theeffect of NaCN treatment in combination with conventional RT and FLASH RT wastested using clonogenic assays on the H460 cell line. Treatment protocols to achieve80% cell viability were found for all four compounds and cell lines. For almost all celllines a treatment protocol to achieve 50% viability was found. A significant increase ofviability of about 50% was detected for combining NaCN treatment with FLASH RT.Further studies on the effects of the compounds in combination with RT have to be doneas well as on their effect on the SOD levels.
000177454 536__ $$0G:(DE-HGF)POF4-315$$a315 - Bildgebung und Radioonkologie (POF4-315)$$cPOF4-315$$fPOF IV$$x0
000177454 909CO $$ooai:inrepo02.dkfz.de:177454$$pVDB
000177454 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)0ba9f9aa0f67731222213b440c10ab5c$$aDeutsches Krebsforschungszentrum$$b0$$kDKFZ
000177454 9131_ $$0G:(DE-HGF)POF4-315$$1G:(DE-HGF)POF4-310$$2G:(DE-HGF)POF4-300$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lKrebsforschung$$vBildgebung und Radioonkologie$$x0
000177454 9141_ $$y2021
000177454 9201_ $$0I:(DE-He78)E041-20160331$$kE041$$lE041 Medizinische Physik in der Radioonkologie$$x0
000177454 980__ $$abachelor
000177454 980__ $$aVDB
000177454 980__ $$aI:(DE-He78)E041-20160331
000177454 980__ $$aUNRESTRICTED