Bachelor Thesis

DKFZ-2021-02546

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Toxicity studies on lung and pancreatic cancer celllines: An approach towards FLASH mechanisms

Wüllner, A.DKFZ*

2021

Abstract: Several studies have hinted towards involvement of radical scavengers in the sparingof healthy tissue in radiotherapy (RT) for ultra-high dose rates (FLASH effect). Thesaturation of the intracellular enzyme Superoxide Dismutase (SOD) is proposed to playa key role in the FLASH effect. The aim of the current study was to establish treatmentprotocols to achieve 80% cell viability of two cancer cell lines for four compounds thataffect the enzyme and its substrate Superoxide. Cytotoxicity assays were done on theH460 lung cancer cell line and the Panc-1 pancreatic cancer cell line with the compoundsH2O2, MSB, NaCN and KCN. The effect of Catalase on cell viability was tested and theeffect of NaCN treatment in combination with conventional RT and FLASH RT wastested using clonogenic assays on the H460 cell line. Treatment protocols to achieve80% cell viability were found for all four compounds and cell lines. For almost all celllines a treatment protocol to achieve 50% viability was found. A significant increase ofviability of about 50% was detected for combining NaCN treatment with FLASH RT.Further studies on the effects of the compounds in combination with RT have to be doneas well as on their effect on the SOD levels.

Note: Corresponding author J. Seco
Note: Bachelorarbeit, Universität Heidelberg, 2021

---

Contributing Institute(s):

1. E041 Medizinische Physik in der Radioonkologie (E041)

Research Program(s):

1. 315 - Bildgebung und Radioonkologie (POF4-315) (POF4-315)

Appears in the scientific report 2021

---