TY  - JOUR
AU  - Fresnais, Margaux
AU  - Liang, Siwen
AU  - Breitkopf, Marius
AU  - Lindner, Joshua Raoul
AU  - Claude, Emmanuelle
AU  - Pringle, Steven
AU  - Levkin, Pavel A
AU  - Demir, Konstantin
AU  - Benzel, Julia
AU  - Sundheimer, Julia
AU  - Statz, Britta
AU  - Pajtler, Kristian
AU  - Pfister, Stefan
AU  - Haefeli, Walter E
AU  - Burhenne, Jürgen
AU  - Longuespée, Rémi
TI  - Analytical Performance Evaluation of New DESI Enhancements for Targeted Drug Quantification in Tissue Sections.
JO  - Pharmaceuticals
VL  - 15
IS  - 6
SN  - 1424-8247
CY  - Basel
PB  - MDPI
M1  - DKFZ-2022-01346
SP  - 694
PY  - 2022
AB  - Desorption/ionization (DI)-mass spectrometric (MS) methods offer considerable advantages of rapidity and low-sample input for the analysis of solid biological matrices such as tissue sections. The concept of desorption electrospray ionization (DESI) offers the possibility to ionize compounds from solid surfaces at atmospheric pressure, without the addition of organic compounds to initiate desorption. However, severe drawbacks from former DESI hardware stability made the development of assays for drug quantification difficult. In the present study, the potential of new prototype source setups (High Performance DESI Sprayer and Heated Transfer Line) for the development of drug quantification assays in tissue sections was evaluated. It was demonstrated that following dedicated optimization, new DESI XS enhancements present promising options regarding targeted quantitative analyses. As a model compound for these developments, ulixertinib, an inhibitor of extracellular signal-regulated kinase (ERK) 1 and 2 was used.
KW  - desorption electrospray ionization (Other)
KW  - drug (Other)
KW  - mass spectrometry (Other)
KW  - profiling (Other)
KW  - quantification (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:35745613
DO  - DOI:10.3390/ph15060694
UR  - https://inrepo02.dkfz.de/record/180467
ER  -