Journal Article

DKFZ-2022-01346

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Analytical Performance Evaluation of New DESI Enhancements for Targeted Drug Quantification in Tissue Sections.

Fresnais, M. ; Liang, S. ; Breitkopf, M. ; Lindner, J. R. ; Claude, E. ; Pringle, S. ; Levkin, P. A. ; Demir, K. ; Benzel, J.DKFZ* ; Sundheimer, J.DKFZ* ; Statz, B.DKFZ* ; Pajtler, K.DKFZ* ; Pfister, S.DKFZ* ; Haefeli, W. E. ; Burhenne, J. ; Longuespée, R.

2022
MDPI Basel

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Please use a persistent id in citations: doi:10.3390/ph15060694

Abstract: Desorption/ionization (DI)-mass spectrometric (MS) methods offer considerable advantages of rapidity and low-sample input for the analysis of solid biological matrices such as tissue sections. The concept of desorption electrospray ionization (DESI) offers the possibility to ionize compounds from solid surfaces at atmospheric pressure, without the addition of organic compounds to initiate desorption. However, severe drawbacks from former DESI hardware stability made the development of assays for drug quantification difficult. In the present study, the potential of new prototype source setups (High Performance DESI Sprayer and Heated Transfer Line) for the development of drug quantification assays in tissue sections was evaluated. It was demonstrated that following dedicated optimization, new DESI XS enhancements present promising options regarding targeted quantitative analyses. As a model compound for these developments, ulixertinib, an inhibitor of extracellular signal-regulated kinase (ERK) 1 and 2 was used.

Keyword(s): desorption electrospray ionization ; drug ; mass spectrometry ; profiling ; quantification

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Contributing Institute(s):

1. B062 Pädiatrische Neuroonkologie (B062)
2. DKTK HD zentral (HD01)

Research Program(s):

1. 312 - Funktionelle und strukturelle Genomforschung (POF4-312) (POF4-312)

Appears in the scientific report 2022

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Database coverage:
; ; ; Article Processing Charges ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; DOAJ Seal ; Ebsco Academic Search ; Essential Science Indicators ; Fees ; IF >= 5 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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