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000182336 0247_ $$2doi$$a10.1002/eji.202249816
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000182336 041__ $$aEnglish
000182336 082__ $$a610
000182336 1001_ $$aLutz, Manfred B$$b0
000182336 245__ $$aGuidelines for mouse and human DC generation.
000182336 260__ $$aWeinheim$$bWiley-VCH$$c2023
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000182336 500__ $$a2023 Nov;53(11):e2249816
000182336 520__ $$aThis article is part of the Dendritic Cell Guidelines article series, which provides a collection of state-of-the-art protocols for the preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy, and functional characterization of mouse and human dendritic cells (DC) from lymphoid organs and various non-lymphoid tissues. This article provides protocols with top ticks and pitfalls for preparation and successful generation of mouse and human DC from different cellular sources, such as murine BM and HoxB8 cells, as well as human CD34+ cells from cord blood, BM, and peripheral blood or peripheral blood monocytes. We describe murine cDC1, cDC2, and pDC generation with Flt3L and the generation of BM-derived DC with GM-CSF. Protocols for human DC generation focus on CD34+ cell culture on OP9 cell layers for cDC1, cDC2, cDC3, and pDC subset generation and DC generation from peripheral blood monocytes (MoDC). Additional protocols include enrichment of murine DC subsets, CRISPR/Cas9 editing, and clinical grade human DC generation. While all protocols were written by experienced scientists who routinely use them in their work, this article was also peer-reviewed by leading experts and approved by all co-authors, making it an essential resource for basic and clinical DC immunologists.
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000182336 650_7 $$2Other$$aDendritic cells
000182336 650_7 $$2Other$$aGeneration
000182336 650_7 $$2Other$$aIn vitro
000182336 650_7 $$2Other$$aIsolation
000182336 7001_ $$aAli, Shafaqat$$b1
000182336 7001_ $$aAudiger, Cindy$$b2
000182336 7001_ $$0P:(DE-He78)d3dbba28fe1239effd15962787cbc363$$aAutenrieth, Stella$$b3$$udkfz
000182336 7001_ $$aBerod, Luciana$$b4
000182336 7001_ $$aBigley, Venetia$$b5
000182336 7001_ $$aCyran, Laura$$b6
000182336 7001_ $$aDalod, Marc$$b7
000182336 7001_ $$aDörrie, Jan$$b8
000182336 7001_ $$aDudziak, Diana$$b9
000182336 7001_ $$aFlórez-Grau, Georgina$$b10
000182336 7001_ $$aGiusiano, Lucila$$b11
000182336 7001_ $$aGodoy, Gloria J$$b12
000182336 7001_ $$aHeuer, Marion$$b13
000182336 7001_ $$aKrug, Anne B$$b14
000182336 7001_ $$aLehmann, Christian H K$$b15
000182336 7001_ $$aMayer, Christian T$$b16
000182336 7001_ $$aNaik, Shalin H$$b17
000182336 7001_ $$aScheu, Stefanie$$b18
000182336 7001_ $$aSchreibelt, Gerty$$b19
000182336 7001_ $$aSegura, Elodie$$b20
000182336 7001_ $$aSeré, Kristin$$b21
000182336 7001_ $$aSparwasser, Tim$$b22
000182336 7001_ $$aTel, Jurjen$$b23
000182336 7001_ $$aXu, Huaming$$b24
000182336 7001_ $$aZenke, Martin$$b25
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