Journal Article

DKFZ-2022-02579

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Guidelines for mouse and human DC generation.

Lutz, M. B. ; Ali, S. ; Audiger, C. ; Autenrieth, S.DKFZ* ; Berod, L. ; Bigley, V. ; Cyran, L. ; Dalod, M. ; Dörrie, J. ; Dudziak, D. ; Flórez-Grau, G. ; Giusiano, L. ; Godoy, G. J. ; Heuer, M. ; Krug, A. B. ; Lehmann, C. H. K. ; Mayer, C. T. ; Naik, S. H. ; Scheu, S. ; Schreibelt, G. ; Segura, E. ; Seré, K. ; Sparwasser, T. ; Tel, J. ; Xu, H. ; Zenke, M.

2023
Wiley-VCH Weinheim

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Please use a persistent id in citations: doi:10.1002/eji.202249816

Abstract: This article is part of the Dendritic Cell Guidelines article series, which provides a collection of state-of-the-art protocols for the preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy, and functional characterization of mouse and human dendritic cells (DC) from lymphoid organs and various non-lymphoid tissues. This article provides protocols with top ticks and pitfalls for preparation and successful generation of mouse and human DC from different cellular sources, such as murine BM and HoxB8 cells, as well as human CD34+ cells from cord blood, BM, and peripheral blood or peripheral blood monocytes. We describe murine cDC1, cDC2, and pDC generation with Flt3L and the generation of BM-derived DC with GM-CSF. Protocols for human DC generation focus on CD34+ cell culture on OP9 cell layers for cDC1, cDC2, cDC3, and pDC subset generation and DC generation from peripheral blood monocytes (MoDC). Additional protocols include enrichment of murine DC subsets, CRISPR/Cas9 editing, and clinical grade human DC generation. While all protocols were written by experienced scientists who routinely use them in their work, this article was also peer-reviewed by leading experts and approved by all co-authors, making it an essential resource for basic and clinical DC immunologists.

Keyword(s): Dendritic cells ; Generation ; In vitro ; Isolation

Note: 2023 Nov;53(11):e2249816

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Contributing Institute(s):

1. Dentritische Zellen bei Infektionen und Krebs (F171)

Research Program(s):

1. 316 - Infektionen, Entzündung und Krebs (POF4-316) (POF4-316)

Appears in the scientific report 2022

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Database coverage:
; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; DEAL Wiley ; Essential Science Indicators ; IF >= 5 ; JCR ; Nationallizenz ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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