TY  - JOUR
AU  - Lutz, Manfred B
AU  - Ali, Shafaqat
AU  - Audiger, Cindy
AU  - Autenrieth, Stella
AU  - Berod, Luciana
AU  - Bigley, Venetia
AU  - Cyran, Laura
AU  - Dalod, Marc
AU  - Dörrie, Jan
AU  - Dudziak, Diana
AU  - Flórez-Grau, Georgina
AU  - Giusiano, Lucila
AU  - Godoy, Gloria J
AU  - Heuer, Marion
AU  - Krug, Anne B
AU  - Lehmann, Christian H K
AU  - Mayer, Christian T
AU  - Naik, Shalin H
AU  - Scheu, Stefanie
AU  - Schreibelt, Gerty
AU  - Segura, Elodie
AU  - Seré, Kristin
AU  - Sparwasser, Tim
AU  - Tel, Jurjen
AU  - Xu, Huaming
AU  - Zenke, Martin
TI  - Guidelines for mouse and human DC generation.
JO  - European journal of immunology
VL  - 53
IS  - 11
SN  - 0014-2980
CY  - Weinheim
PB  - Wiley-VCH
M1  - DKFZ-2022-02579
SP  - e2249816
PY  - 2023
N1  - 2023 Nov;53(11):e2249816
AB  - This article is part of the Dendritic Cell Guidelines article series, which provides a collection of state-of-the-art protocols for the preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy, and functional characterization of mouse and human dendritic cells (DC) from lymphoid organs and various non-lymphoid tissues. This article provides protocols with top ticks and pitfalls for preparation and successful generation of mouse and human DC from different cellular sources, such as murine BM and HoxB8 cells, as well as human CD34+ cells from cord blood, BM, and peripheral blood or peripheral blood monocytes. We describe murine cDC1, cDC2, and pDC generation with Flt3L and the generation of BM-derived DC with GM-CSF. Protocols for human DC generation focus on CD34+ cell culture on OP9 cell layers for cDC1, cDC2, cDC3, and pDC subset generation and DC generation from peripheral blood monocytes (MoDC). Additional protocols include enrichment of murine DC subsets, CRISPR/Cas9 editing, and clinical grade human DC generation. While all protocols were written by experienced scientists who routinely use them in their work, this article was also peer-reviewed by leading experts and approved by all co-authors, making it an essential resource for basic and clinical DC immunologists.
KW  - Dendritic cells (Other)
KW  - Generation (Other)
KW  - In vitro (Other)
KW  - Isolation (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:36303448
DO  - DOI:10.1002/eji.202249816
UR  - https://inrepo02.dkfz.de/record/182336
ER  -