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@ARTICLE{Lutz:182336,
      author       = {M. B. Lutz and S. Ali and C. Audiger and S. Autenrieth$^*$
                      and L. Berod and V. Bigley and L. Cyran and M. Dalod and J.
                      Dörrie and D. Dudziak and G. Flórez-Grau and L. Giusiano
                      and G. J. Godoy and M. Heuer and A. B. Krug and C. H. K.
                      Lehmann and C. T. Mayer and S. H. Naik and S. Scheu and G.
                      Schreibelt and E. Segura and K. Seré and T. Sparwasser and
                      J. Tel and H. Xu and M. Zenke},
      title        = {{G}uidelines for mouse and human {DC} generation.},
      journal      = {European journal of immunology},
      volume       = {53},
      number       = {11},
      issn         = {0014-2980},
      address      = {Weinheim},
      publisher    = {Wiley-VCH},
      reportid     = {DKFZ-2022-02579},
      pages        = {e2249816},
      year         = {2023},
      note         = {2023 Nov;53(11):e2249816},
      abstract     = {This article is part of the Dendritic Cell Guidelines
                      article series, which provides a collection of
                      state-of-the-art protocols for the preparation, phenotype
                      analysis by flow cytometry, generation, fluorescence
                      microscopy, and functional characterization of mouse and
                      human dendritic cells (DC) from lymphoid organs and various
                      non-lymphoid tissues. This article provides protocols with
                      top ticks and pitfalls for preparation and successful
                      generation of mouse and human DC from different cellular
                      sources, such as murine BM and HoxB8 cells, as well as human
                      CD34+ cells from cord blood, BM, and peripheral blood or
                      peripheral blood monocytes. We describe murine cDC1, cDC2,
                      and pDC generation with Flt3L and the generation of
                      BM-derived DC with GM-CSF. Protocols for human DC generation
                      focus on CD34+ cell culture on OP9 cell layers for cDC1,
                      cDC2, cDC3, and pDC subset generation and DC generation from
                      peripheral blood monocytes (MoDC). Additional protocols
                      include enrichment of murine DC subsets, CRISPR/Cas9
                      editing, and clinical grade human DC generation. While all
                      protocols were written by experienced scientists who
                      routinely use them in their work, this article was also
                      peer-reviewed by leading experts and approved by all
                      co-authors, making it an essential resource for basic and
                      clinical DC immunologists.},
      keywords     = {Dendritic cells (Other) / Generation (Other) / In vitro
                      (Other) / Isolation (Other)},
      cin          = {F171},
      ddc          = {610},
      cid          = {I:(DE-He78)F171-20160331},
      pnm          = {316 - Infektionen, Entzündung und Krebs (POF4-316)},
      pid          = {G:(DE-HGF)POF4-316},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:36303448},
      doi          = {10.1002/eji.202249816},
      url          = {https://inrepo02.dkfz.de/record/182336},
}