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@ARTICLE{Karasu:182368,
author = {O. R. Karasu and A. Neuner and E. S. Atorino and G.
Pereira$^*$ and E. Schiebel},
title = {{T}he central scaffold protein {CEP}350 coordinates
centriole length, stability, and maturation.},
journal = {The journal of cell biology},
volume = {221},
number = {12},
issn = {0021-9525},
address = {New York, NY},
publisher = {Rockefeller Univ. Press},
reportid = {DKFZ-2022-02607},
pages = {e202203081},
year = {2022},
note = {DKFZ-ZMBH Alliance},
abstract = {The centriole is the microtubule-based backbone that
ensures integrity, function, and cell cycle-dependent
duplication of centrosomes. Mostly unclear mechanisms
control structural integrity of centrioles. Here, we show
that the centrosome protein CEP350 functions as scaffold
that coordinates distal-end properties of centrioles such as
length, stability, and formation of distal and subdistal
appendages. CEP350 fulfills these diverse functions by
ensuring centriolar localization of WDR90, recruiting the
proteins CEP78 and OFD1 to the distal end of centrioles and
promoting the assembly of subdistal appendages that have a
role in removing the daughter-specific protein Centrobin.
The CEP350-FOP complex in association with CEP78 or OFD1
controls centriole microtubule length. Centrobin safeguards
centriole distal end stability, especially in the
compromised CEP350-/- cells, while the CEP350-FOP-WDR90 axis
secures centriole integrity. This study identifies CEP350 as
a guardian of the distal-end region of centrioles without
having an impact on the proximal PCM part.},
keywords = {Centrioles: metabolism / Cell Cycle Proteins: genetics /
Cell Cycle Proteins: metabolism / Microtubule-Associated
Proteins: genetics / Microtubule-Associated Proteins:
metabolism / Centrosome: metabolism / Microtubules: genetics
/ Microtubules: metabolism / Cell Cycle Proteins (NLM
Chemicals) / Microtubule-Associated Proteins (NLM
Chemicals)},
cin = {A180},
ddc = {570},
cid = {I:(DE-He78)A180-20160331},
pnm = {311 - Zellbiologie und Tumorbiologie (POF4-311)},
pid = {G:(DE-HGF)POF4-311},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:36315013},
doi = {10.1083/jcb.202203081},
url = {https://inrepo02.dkfz.de/record/182368},
}