Journal Article

DKFZ-2022-02607

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png The central scaffold protein CEP350 coordinates centriole length, stability, and maturation.

Karasu, O. R. ; Neuner, A. ; Atorino, E. S. ; Pereira, G.DKFZ* ; Schiebel, E.

2022
Rockefeller Univ. Press New York, NY

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Please use a persistent id in citations: doi:10.1083/jcb.202203081

Abstract: The centriole is the microtubule-based backbone that ensures integrity, function, and cell cycle-dependent duplication of centrosomes. Mostly unclear mechanisms control structural integrity of centrioles. Here, we show that the centrosome protein CEP350 functions as scaffold that coordinates distal-end properties of centrioles such as length, stability, and formation of distal and subdistal appendages. CEP350 fulfills these diverse functions by ensuring centriolar localization of WDR90, recruiting the proteins CEP78 and OFD1 to the distal end of centrioles and promoting the assembly of subdistal appendages that have a role in removing the daughter-specific protein Centrobin. The CEP350-FOP complex in association with CEP78 or OFD1 controls centriole microtubule length. Centrobin safeguards centriole distal end stability, especially in the compromised CEP350-/- cells, while the CEP350-FOP-WDR90 axis secures centriole integrity. This study identifies CEP350 as a guardian of the distal-end region of centrioles without having an impact on the proximal PCM part.

Keyword(s): Centrioles: metabolism (MeSH) ; Cell Cycle Proteins: genetics (MeSH) ; Cell Cycle Proteins: metabolism (MeSH) ; Microtubule-Associated Proteins: genetics (MeSH) ; Microtubule-Associated Proteins: metabolism (MeSH) ; Centrosome: metabolism (MeSH) ; Microtubules: genetics (MeSH) ; Microtubules: metabolism (MeSH) ; Cell Cycle Proteins ; Microtubule-Associated Proteins

Note: DKFZ-ZMBH Alliance

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Contributing Institute(s):

1. A180 Molecular Biology of Centrosomes (A180)

Research Program(s):

1. 311 - Zellbiologie und Tumorbiologie (POF4-311) (POF4-311)

Appears in the scientific report 2022

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Database coverage:
; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 5 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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