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@ARTICLE{Vieira:186452,
author = {J. R. Vieira and B. Shah and S. Dupraz and I. Paredes and
P. Himmels and G. Schermann and H. Adler and A. Motta and L.
Gärtner and A. Navarro-Aragall and E. Ioannou and E.
Dyukova and R. Bonnavion and A. Fischer$^*$ and D. Bonanomi
and F. Bradke and C. Ruhrberg and C. Ruiz de Almodóvar},
title = {{E}ndothelial {P}lexin{D}1 signaling instructs spinal cord
vascularization and motor neuron development.},
journal = {Neuron},
volume = {110},
number = {24},
issn = {0896-6273},
address = {New York, NY},
publisher = {Elsevier},
reportid = {DKFZ-2022-03173},
pages = {4074 - 4089.e6},
year = {2022},
abstract = {How the vascular and neural compartment cooperate to
achieve such a complex and highly specialized structure as
the central nervous system is still unclear. Here, we reveal
a crosstalk between motor neurons (MNs) and endothelial
cells (ECs), necessary for the coordinated development of
MNs. By analyzing cell-to-cell interaction profiles of the
mouse developing spinal cord, we uncovered semaphorin 3C
(Sema3C) and PlexinD1 as a communication axis between MNs
and ECs. Using cell-specific knockout mice and in vitro
assays, we demonstrate that removal of Sema3C in MNs, or its
receptor PlexinD1 in ECs, results in premature and aberrant
vascularization of MN columns. Those vascular defects impair
MN axon exit from the spinal cord. Impaired PlexinD1
signaling in ECs also causes MN maturation defects at later
stages. This study highlights the importance of a timely and
spatially controlled communication between MNs and ECs for
proper spinal cord development.},
keywords = {Animals / Mice / Endothelial Cells / Motor Neurons:
physiology / Spinal Cord / Signal Transduction / Axons /
Mice, Knockout / CNS vascularization (Other) / PlexinD1
(Other) / Sema3C (Other) / blood vessel (Other) /
endothelial cell (Other) / motor neuron (Other) /
neuro-vascular communication (Other) / neurodevelopment
(Other) / spinal cord (Other)},
cin = {A270},
ddc = {610},
cid = {I:(DE-He78)A270-20160331},
pnm = {311 - Zellbiologie und Tumorbiologie (POF4-311)},
pid = {G:(DE-HGF)POF4-311},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:36549270},
doi = {10.1016/j.neuron.2022.12.005},
url = {https://inrepo02.dkfz.de/record/186452},
}