Journal Article

DKFZ-2022-03173

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Endothelial PlexinD1 signaling instructs spinal cord vascularization and motor neuron development.

Vieira, J. R. ; Shah, B. ; Dupraz, S. ; Paredes, I. ; Himmels, P. ; Schermann, G. ; Adler, H. ; Motta, A. ; Gärtner, L. ; Navarro-Aragall, A. ; Ioannou, E. ; Dyukova, E. ; Bonnavion, R. ; Fischer, A.DKFZ* ; Bonanomi, D. ; Bradke, F. ; Ruhrberg, C. ; Ruiz de Almodóvar, C.

2022
Elsevier New York, NY

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Please use a persistent id in citations: doi:10.1016/j.neuron.2022.12.005

Abstract: How the vascular and neural compartment cooperate to achieve such a complex and highly specialized structure as the central nervous system is still unclear. Here, we reveal a crosstalk between motor neurons (MNs) and endothelial cells (ECs), necessary for the coordinated development of MNs. By analyzing cell-to-cell interaction profiles of the mouse developing spinal cord, we uncovered semaphorin 3C (Sema3C) and PlexinD1 as a communication axis between MNs and ECs. Using cell-specific knockout mice and in vitro assays, we demonstrate that removal of Sema3C in MNs, or its receptor PlexinD1 in ECs, results in premature and aberrant vascularization of MN columns. Those vascular defects impair MN axon exit from the spinal cord. Impaired PlexinD1 signaling in ECs also causes MN maturation defects at later stages. This study highlights the importance of a timely and spatially controlled communication between MNs and ECs for proper spinal cord development.

Keyword(s): Animals (MeSH) ; Mice (MeSH) ; Endothelial Cells (MeSH) ; Motor Neurons: physiology (MeSH) ; Spinal Cord (MeSH) ; Signal Transduction (MeSH) ; Axons (MeSH) ; Mice, Knockout (MeSH) ; CNS vascularization ; PlexinD1 ; Sema3C ; blood vessel ; endothelial cell ; motor neuron ; neuro-vascular communication ; neurodevelopment ; spinal cord

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Contributing Institute(s):

1. A270 Vaskuläre Signaltransduktion und Krebs (A270)

Research Program(s):

1. 311 - Zellbiologie und Tumorbiologie (POF4-311) (POF4-311)

Appears in the scientific report 2022

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Database coverage:
; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 15 ; JCR ; Nationallizenz ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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