% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Pagliari:186474,
      author       = {F. Pagliari$^*$ and E. Sogne and D. Panella and G.
                      Perozziello and C. Liberale and G. Das and A. Turdo and S.
                      Di Franco and J. Seco$^*$ and A. Falqui and S. Gratteri and
                      A. Pujia and E. Di Fabrizio and P. Candeloro and L.
                      Tirinato$^*$},
      title        = {{C}orrelative {R}aman-{E}lectron-{L}ight ({CREL})
                      {M}icroscopy {A}nalysis of {L}ipid {D}roplets in {M}elanoma
                      {C}ancer {S}tem {C}ells.},
      journal      = {Biosensors},
      volume       = {12},
      number       = {12},
      issn         = {2079-6374},
      address      = {Basel},
      publisher    = {MDPI},
      reportid     = {DKFZ-2022-03195},
      pages        = {1102},
      year         = {2022},
      note         = {#EA:E041#LA:E041#},
      abstract     = {Among all neoplasms, melanoma is characterized by a very
                      high percentage of cancer stem cells (CSCs). Several markers
                      have been proposed for their identification, and lipid
                      droplets (LDs) are among them. Different techniques are used
                      for their characterization such as mass spectrometry,
                      imaging techniques, and vibrational spectroscopies. Some
                      emerging experimental approaches for the study of LDs are
                      represented by correlative light-electron microscopy and by
                      correlative Raman imaging-scanning electron microscopy
                      (SEM). Based on these scientific approaches, we developed a
                      novel methodology (CREL) by combining Raman
                      micro-spectroscopy, confocal fluorescence microscopy, and
                      SEM coupled with an energy-dispersive X-ray spectroscopy
                      module. This procedure correlated cellular morphology,
                      chemical properties, and spatial distribution from the same
                      region of interest, and in this work, we presented the
                      application of CREL for the analysis of LDs within
                      patient-derived melanoma CSCs (MCSCs).},
      keywords     = {Humans / Lipid Droplets / Electrons / Microscopy, Electron,
                      Scanning / Spectrum Analysis, Raman: methods / Melanoma /
                      Neoplastic Stem Cells / Raman micro-imaging (Other) /
                      correlative microscopy (Other) / electron microscopy (Other)
                      / lipid droplets (Other) / melanoma cancer stem cells
                      (Other)},
      cin          = {E041},
      ddc          = {570},
      cid          = {I:(DE-He78)E041-20160331},
      pnm          = {315 - Bildgebung und Radioonkologie (POF4-315)},
      pid          = {G:(DE-HGF)POF4-315},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:36551069},
      pmc          = {pmc:PMC9776032},
      doi          = {10.3390/bios12121102},
      url          = {https://inrepo02.dkfz.de/record/186474},
}