Journal Article

DKFZ-2022-03195

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Correlative Raman-Electron-Light (CREL) Microscopy Analysis of Lipid Droplets in Melanoma Cancer Stem Cells.

Pagliari, F. (First author)DKFZ* ; Sogne, E. ; Panella, D. ; Perozziello, G. ; Liberale, C. ; Das, G. ; Turdo, A. ; Di Franco, S. ; Seco, J.DKFZ* ; Falqui, A. ; Gratteri, S. ; Pujia, A. ; Di Fabrizio, E. ; Candeloro, P. ; Tirinato, L. (Last author)DKFZ*

2022
MDPI Basel

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Please use a persistent id in citations: doi:10.3390/bios12121102

Abstract: Among all neoplasms, melanoma is characterized by a very high percentage of cancer stem cells (CSCs). Several markers have been proposed for their identification, and lipid droplets (LDs) are among them. Different techniques are used for their characterization such as mass spectrometry, imaging techniques, and vibrational spectroscopies. Some emerging experimental approaches for the study of LDs are represented by correlative light-electron microscopy and by correlative Raman imaging-scanning electron microscopy (SEM). Based on these scientific approaches, we developed a novel methodology (CREL) by combining Raman micro-spectroscopy, confocal fluorescence microscopy, and SEM coupled with an energy-dispersive X-ray spectroscopy module. This procedure correlated cellular morphology, chemical properties, and spatial distribution from the same region of interest, and in this work, we presented the application of CREL for the analysis of LDs within patient-derived melanoma CSCs (MCSCs).

Keyword(s): Humans (MeSH) ; Lipid Droplets (MeSH) ; Electrons (MeSH) ; Microscopy, Electron, Scanning (MeSH) ; Spectrum Analysis, Raman: methods (MeSH) ; Melanoma (MeSH) ; Neoplastic Stem Cells (MeSH) ; Raman micro-imaging ; correlative microscopy ; electron microscopy ; lipid droplets ; melanoma cancer stem cells

Note: #EA:E041#LA:E041#

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Contributing Institute(s):

1. E041 Med. Physik in der Radioonkologie (E041)

Research Program(s):

1. 315 - Bildgebung und Radioonkologie (POF4-315) (POF4-315)

Appears in the scientific report 2022

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