%0 Journal Article
%A Thürmann, Loreen
%A Klös, Matthias
%A Mackowiak, Sebastian D
%A Bieg, Matthias
%A Bauer, Tobias
%A Ishaque, Naveed
%A Messingschlager, Marey
%A Herrmann, Carl
%A Röder, Stefan
%A Bauer, Mario
%A Schäuble, Sascha
%A Faessler, Erik
%A Hahn, Udo
%A Weichenhan, Dieter
%A Mücke, Oliver
%A Plass, Christoph
%A Borte, Michael
%A von Mutius, Erika
%A Stangl, Gabriele I
%A Lauener, Roger
%A Karvonen, Anne M
%A Divaret-Chauveau, Amandine
%A Riedler, Josef
%A Heinrich, Joachim
%A Standl, Marie
%A von Berg, Andrea
%A Schaaf, Beate
%A Herberth, Gunda
%A Kabesch, Michael
%A Eils, Roland
%A Trump, Saskia
%A Lehmann, Irina
%T Global hypomethylation in childhood asthma identified by genome-wide DNA-methylation sequencing preferentially affects enhancer regions.
%J Allergy
%V 78
%N 6
%@ 0105-4538
%C Oxford
%I Wiley
%M DKFZ-2023-00234
%P 1489-1506
%D 2023
%Z 2023 Jun;78(6):1489-1506
%X Childhood asthma is a result of a complex interaction of genetic and environmental components causing epigenetic and immune dysregulation, airway inflammation and impaired lung function. Although different microarray based EWAS studies have been conducted, the impact of epigenetic regulation in asthma development is still widely unknown. We have therefore applied unbiased whole genome bisulfite sequencing (WGBS) to characterize global DNA-methylation profiles of asthmatic children compared to healthy controls.Peripheral blood samples of 40 asthmatic and 42 control children aged 5-15 years from three birth cohorts were sequenced together with paired cord blood samples. Identified differentially methylated regions (DMRs) were categorized in genotype-associated, cell-type-dependent, or prenatally-primed. Network analysis and subsequent natural language processing of DMR-associated genes was complemented by targeted analysis of functional translation of epigenetic regulation on the transcriptional and protein level.In total, 158 DMRs were identified in asthmatic children compared to controls of which 37
%K DNA-methylation (Other)
%K asthma (Other)
%K cord blood (Other)
%K prenatal exposure (Other)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:36704932
%R 10.1111/all.15658
%U https://inrepo02.dkfz.de/record/212549