Journal Article

DKFZ-2023-00595

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Scalable RT-LAMP-based SARS-CoV-2 testing for infection surveillance with applications in pandemic preparedness.

Lou, D. ; Meurer, M. ; Ovchinnikova, S. ; Burk, R. ; Denzler, A. ; Herbst, K. ; Papaioannou, I. A. ; Duan, Y. ; Jacobs, M. L. ; Witte, V. ; Ürge, D. ; Kirrmaier, D.DKFZ* ; Krogemann, M. ; Gubicza, K.DKFZ* ; Boerner, K. ; Bundschuh, C. ; Weidner, N. M. ; Merle, U. ; Knorr, B. ; Welker, A. ; Denkinger, C. M. ; Schnitzler, P. ; Kräusslich, H.-G. ; Dao Thi, V. L. ; De Allegri, M. ; Nguyen, H. T. ; Deckert, A. ; Anders, S. ; Knop, M. (Last author)DKFZ*

2023
Wiley Hoboken, NJ [u.a.]

This record in other databases:  

Please use a persistent id in citations: doi:10.15252/embr.202357162

Abstract: Throughout the SARS-CoV-2 pandemic, limited diagnostic capacities prevented sentinel testing, demonstrating the need for novel testing infrastructures. Here, we describe the setup of a cost-effective platform that can be employed in a high-throughput manner, which allows surveillance testing as an acute pandemic control and preparedness tool, exemplified by SARS-CoV-2 diagnostics in an academic environment. The strategy involves self-sampling based on gargling saline, pseudonymized sample handling, automated RNA extraction, and viral RNA detection using a semiquantitative multiplexed colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay with an analytical sensitivity comparable with RT-qPCR. We provide standard operating procedures and an integrated software solution for all workflows, including sample logistics, analysis by colorimetry or sequencing, and communication of results. We evaluated factors affecting the viral load and the stability of gargling samples as well as the diagnostic sensitivity of the RT-LAMP assay. In parallel, we estimated the economic costs of setting up and running the test station. We performed > 35,000 tests, with an average turnover time of < 6 h from sample arrival to result announcement. Altogether, our work provides a blueprint for fast, sensitive, scalable, cost- and labor-efficient RT-LAMP diagnostics, which is independent of potentially limiting clinical diagnostics supply chains.

Keyword(s): COVID-19 tests ; RT-LAMP ; corona tests ; pandemic preparedness ; surveillance testing

Note: #LA:A260# / 2023 May 4;24(5):e57162

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Contributing Institute(s):

1. A260 Zellmorphogenese und Signalübermittlung (A260)

Research Program(s):

1. 311 - Zellbiologie und Tumorbiologie (POF4-311) (POF4-311)

Appears in the scientific report 2023

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Database coverage:
; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; DEAL Wiley ; Essential Science Indicators ; IF >= 5 ; JCR ; PubMed Central ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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