TY  - JOUR
AU  - Stögbauer, Fabian
AU  - Beck, Susanne
AU  - Ourailidis, Iordanis
AU  - Hess, Jochen
AU  - Poremba, Christopher
AU  - Lauterbach, Maren
AU  - Wollenberg, Barbara
AU  - Buchberger, Anna Maria Stefanie
AU  - Jesinghaus, Moritz
AU  - Schirmacher, Peter
AU  - Stenzinger, Albrecht
AU  - Weichert, Wilko
AU  - Boxberg, Melanie
AU  - Budczies, Jan
TI  - Tumour budding-based grading as independent prognostic biomarker in HPV-positive and HPV-negative head and neck cancer.
JO  - British journal of cancer
VL  - 128
IS  - 12
SN  - 0007-0920
CY  - Edinburgh
PB  - Nature Publ. Group
M1  - DKFZ-2023-00738
SP  - 2295-2306
PY  - 2023
N1  - 2023 Jun;128(12):2295-2306
AB  - The prognostic significance of tumour budding (TB) and minimal cell nest size (MCNS) was shown in human papillomavirus (HPV)-negative head and neck squamous cell carcinomas (HNSCC). However, the optimisation of cutpoints, the prognostic impact in HPV-positive HNSCC, and the comparison with other histopathological grading systems are insufficiently investigated.TB and MCNS were analysed digitally in 1 and 10 high-power fields (HPF) of 331 HPV-positive and HPV-negative cases from TCGA. Optimising the cutpoints a new cellular dissociation grading (CDG) system was defined and compared to the WHO grading and the Brandwein-Gensler (BG) risk model.The two-tiered CDG system based solely on TB yielded optimal prognostic stratification with shortened overall survival for CDG-high cases. Optimal cut-offs were two buds (1 HPF) and six buds (10 HPF), respectively. Analysing MCNS did not add prognostic significance to quantifying TB. CDG was a significant prognostic marker in HPV-negative and HPV-positive tumours and prognostically superior to the WHO and BG systems. High CDG was associated with clinically occult lymph-node metastases.The most comprehensive study of TB in HNSCC so far confirmed its prognostic impact in HPV-negative tumours and for the first time in HPV-positive tumours. Further studies are warranted to evaluate its applicability for therapy guidance in HNSCC.
LB  - PUB:(DE-HGF)16
C6  - pmid:37045906
DO  - DOI:10.1038/s41416-023-02240-y
UR  - https://inrepo02.dkfz.de/record/275372
ER  -