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@ARTICLE{Schfer:276873,
author = {M. Schäfer$^*$ and M. Schneider$^*$ and T. Müller$^*$ and
N. Franz$^*$ and I. Braspenning-Wesch$^*$ and S. Stephan$^*$
and G. Schmidt and J. Krijgsveld$^*$ and D. Helm$^*$ and F.
Rösl$^*$ and D. Hasche$^*$},
title = {{S}patial tissue proteomics reveals distinct landscapes of
heterogeneity in cutaneous papillomavirus-induced
keratinocyte carcinomas.},
journal = {Journal of medical virology},
volume = {95},
number = {6},
issn = {0146-6615},
address = {Bognor Regis [u.a.]},
publisher = {Wiley},
reportid = {DKFZ-2023-01182},
pages = {e28850},
year = {2023},
note = {#EA:F030#LA:F030#},
abstract = {Infection with certain cutaneous human papillomaviruses
(HPV), in conjunction with chronic ultraviolet (UV)
exposure, are the major cofactors of non-melanoma skin
cancer (NMSC), the most frequent cancer type worldwide.
Cutaneous squamous cell carcinomas (SCCs) as well as tumors
in general represent three-dimensional entities determined
by both temporal and spatial constraints. Whole tissue
proteomics is a straightforward approach to understand
tumorigenesis in better detail, but studies focusing on
different progression states toward a dedifferentiated SCC
phenotype on a spatial level are rare. Here, we applied an
innovative proteomic workflow on formalin-fixed,
paraffin-embedded (FFPE) epithelial tumors derived from the
preclinical animal model Mastomys coucha. This rodent is
naturally infected with its genuine cutaneous papillomavirus
and closely mimics skin carcinogenesis in the context of
cutaneous HPV infections in humans. We deciphered cellular
networks by comparing diverse epithelial tissues with
respect to their differentiation level and infection status.
Our study reveals novel regulatory proteins and pathways
associated with virus-induced tumor initiation and
progression of SCCs. This approach provides the basis to
better comprehend the multistep process of skin
carcinogenesis.},
keywords = {Mastomys coucha (Other) / MnPV (Other) / cutaneous
papillomavirus (Other) / in vivo proteomics (Other) /
non-melanoma skin cancer (NMSC) (Other)},
cin = {F030 / W120 / B230 / W210},
ddc = {610},
cid = {I:(DE-He78)F030-20160331 / I:(DE-He78)W120-20160331 /
I:(DE-He78)B230-20160331 / I:(DE-He78)W210-20160331},
pnm = {316 - Infektionen, Entzündung und Krebs (POF4-316)},
pid = {G:(DE-HGF)POF4-316},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:37322807},
doi = {10.1002/jmv.28850},
url = {https://inrepo02.dkfz.de/record/276873},
}