Journal Article

DKFZ-2023-01182

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Spatial tissue proteomics reveals distinct landscapes of heterogeneity in cutaneous papillomavirus-induced keratinocyte carcinomas.

Schäfer, M. (First author)DKFZ* ; Schneider, M.DKFZ* ; Müller, T.DKFZ* ; Franz, N.DKFZ* ; Braspenning-Wesch, I.DKFZ* ; Stephan, S.DKFZ* ; Schmidt, G. ; Krijgsveld, J.DKFZ* ; Helm, D.DKFZ* ; Rösl, F.DKFZ* ; Hasche, D. (Last author)DKFZ*

2023
Wiley Bognor Regis [u.a.]

This record in other databases:  

Please use a persistent id in citations: doi:10.1002/jmv.28850

Abstract: Infection with certain cutaneous human papillomaviruses (HPV), in conjunction with chronic ultraviolet (UV) exposure, are the major cofactors of non-melanoma skin cancer (NMSC), the most frequent cancer type worldwide. Cutaneous squamous cell carcinomas (SCCs) as well as tumors in general represent three-dimensional entities determined by both temporal and spatial constraints. Whole tissue proteomics is a straightforward approach to understand tumorigenesis in better detail, but studies focusing on different progression states toward a dedifferentiated SCC phenotype on a spatial level are rare. Here, we applied an innovative proteomic workflow on formalin-fixed, paraffin-embedded (FFPE) epithelial tumors derived from the preclinical animal model Mastomys coucha. This rodent is naturally infected with its genuine cutaneous papillomavirus and closely mimics skin carcinogenesis in the context of cutaneous HPV infections in humans. We deciphered cellular networks by comparing diverse epithelial tissues with respect to their differentiation level and infection status. Our study reveals novel regulatory proteins and pathways associated with virus-induced tumor initiation and progression of SCCs. This approach provides the basis to better comprehend the multistep process of skin carcinogenesis.

Keyword(s): Mastomys coucha ; MnPV ; cutaneous papillomavirus ; in vivo proteomics ; non-melanoma skin cancer (NMSC)

Note: #EA:F030#LA:F030#

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Contributing Institute(s):

1. F030 Virale Transformationsmechanismen (F030)
2. Proteomics (W120)
3. B230 Proteomik von Stammzellen und Krebs (B230)
4. Lichtmikroskopie (W210)

Research Program(s):

1. 316 - Infektionen, Entzündung und Krebs (POF4-316) (POF4-316)

Appears in the scientific report 2023

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Database coverage:
; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Clinical Medicine ; Current Contents - Life Sciences ; DEAL Wiley ; Essential Science Indicators ; IF >= 10 ; JCR ; Nationallizenz ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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