TY  - JOUR
AU  - Johny, Jacob
AU  - van Halteren, Charlotte E R
AU  - Cakir, Fatih-Can
AU  - Zwiehoff, Sandra
AU  - Behrends, Carina
AU  - Bäumer, Christian
AU  - Timmermann, Beate
AU  - Rauschenbach, Laurèl
AU  - Tippelt, Stephan
AU  - Scheffler, Björn
AU  - Schramm, Alexander
AU  - Rehbock, Christoph
AU  - Barcikowski, Stephan
TI  - Surface Chemistry and Specific Surface Area rule the Efficiency of Gold Nanoparticle Sensitizers in Proton Therapy.
JO  - Chemistry - a European journal
VL  - 29
IS  - 50
SN  - 0947-6539
CY  - Weinheim
PB  - Wiley-VCH
M1  - DKFZ-2023-01202
SP  - e202301260
PY  - 2023
N1  - 2023 Sep 6;29(50):e202301260
AB  - Gold nanoparticles (AuNPs) are known radiosensitizers in proton therapy (PT) applicable for the treatment of solid tumors, where they amplify production of reactive oxygen species (ROS). However, it is underexplored how this amplification is correlated with the AuNPs´ surface chemistry. To clarify this issue, we fabricated ligand-free AuNPs of different mean diameters by laser ablation in liquids (LAL) and laser fragmentation in liquids (LFL) and irradiated them with clinically relevant proton fields using water phantoms. ROS generation was monitored by the fluorescent dye 7-OH-coumarin. Our findings reveal an enhancement of ROS production driven by I) increased total particle surface area, II) utilization of ligand-free AuNPs avoiding sodium citrate as a radical quencher ligands, and III) a higher density of structural defects generated by LFL synthesis, indicated by surface charge density. Based on these findings it may be concluded that the surface chemistry is a major and underexplored contributor to ROS generation and sensitizing effects of AuNPs in PT. We further highlight the applicability of AuNPs in vitro in human medulloblastoma cells.
KW  - clinical irradiation dose (Other)
KW  - gold nanoparticle colloid (Other)
KW  - hydroxyl radicals (Other)
KW  - laser processing in liquids (Other)
KW  - proton radiotherapy (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:37334753
DO  - DOI:10.1002/chem.202301260
UR  - https://inrepo02.dkfz.de/record/276909
ER  -