%0 Journal Article
%A Zhang, Junyi
%A Straehle, Jakob
%A Joseph, Kevin
%A Neidert, Nicolas
%A Behringer, Simon
%A Göldner, Jonathan
%A Vlachos, Andreas
%A Prinz, Marco
%A Fung, Christian
%A Beck, Jürgen
%A Schnell, Oliver
%A Heiland, Dieter Henrik
%A Ravi, Vidhya M
%T Isolation and profiling of viable tumor cells from human ex vivo glioblastoma cultures through single-cell transcriptomics.
%J STAR Protocols
%V 4
%N 3
%@ 2666-1667
%C Amsterdam
%I Elsevier
%M DKFZ-2023-01337
%P 102383
%D 2023
%X Single-cell RNA-sequencing (scRNA-seq) is becoming a ubiquitous method in profiling the cellular transcriptomes of both malignant and non-malignant cells from the human brain. Here, we present a protocol to isolate viable tumor cells from human ex vivo glioblastoma cultures for single-cell transcriptomic analysis. We describe steps including surgical tissue collection, sectioning, culturing, primary tumor cells inoculation, growth tracking, fluorescence-based cell sorting, and population-enriched scRNA-seq. This comprehensive methodology empowers in-depth understanding of brain tumor biology at the single-cell level. For complete details on the use and execution of this protocol, please refer to Ravi et al.1.
%K Cancer (Other)
%K Cell Biology (Other)
%K Cell Isolation (Other)
%K Flow Cytometry/Mass Cytometry (Other)
%K Microscopy (Other)
%K Model Organisms (Other)
%K Molecular Biology (Other)
%K RNAseq (Other)
%K Sequencing (Other)
%K Single Cell (Other)
%K Tissue Engineering (Other)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:37393609
%R 10.1016/j.xpro.2023.102383
%U https://inrepo02.dkfz.de/record/277319