Isolation and profiling of viable tumor cells from human ex vivo glioblastoma cultures through single-cell transcriptomics.

Zhang, Junyi; Straehle, Jakob; Prinz, Marco; Behringer, Simon; Joseph, Kevin; Neidert, Nicolas; Beck, Jürgen; Heiland, Dieter Henrik; Ravi, Vidhya M; Göldner, Jonathan; Schnell, Oliver; Fung, Christian; Vlachos, Andreas

doi:10.1016/j.xpro.2023.102383

Journal Article

DKFZ-2023-01337

Isolation and profiling of viable tumor cells from human ex vivo glioblastoma cultures through single-cell transcriptomics.

Zhang, J. ; Straehle, J. ; Joseph, K. ; Neidert, N. ; Behringer, S. ; Göldner, J. ; Vlachos, A. ; Prinz, M. ; Fung, C. ; Beck, J. ; Schnell, O. ; Heiland, D. H.DKFZ* ; Ravi, V. M.

2023
Elsevier Amsterdam

STAR Protocols 4(3), 102383 (2023) [10.1016/j.xpro.2023.102383]

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Abstract: Single-cell RNA-sequencing (scRNA-seq) is becoming a ubiquitous method in profiling the cellular transcriptomes of both malignant and non-malignant cells from the human brain. Here, we present a protocol to isolate viable tumor cells from human ex vivo glioblastoma cultures for single-cell transcriptomic analysis. We describe steps including surgical tissue collection, sectioning, culturing, primary tumor cells inoculation, growth tracking, fluorescence-based cell sorting, and population-enriched scRNA-seq. This comprehensive methodology empowers in-depth understanding of brain tumor biology at the single-cell level. For complete details on the use and execution of this protocol, please refer to Ravi et al.1.

Keyword(s): Cancer ; Cell Biology ; Cell Isolation ; Flow Cytometry/Mass Cytometry ; Microscopy ; Model Organisms ; Molecular Biology ; RNAseq ; Sequencing ; Single Cell ; Tissue Engineering