TY  - JOUR
AU  - Zhang, Junyi
AU  - Straehle, Jakob
AU  - Joseph, Kevin
AU  - Neidert, Nicolas
AU  - Behringer, Simon
AU  - Göldner, Jonathan
AU  - Vlachos, Andreas
AU  - Prinz, Marco
AU  - Fung, Christian
AU  - Beck, Jürgen
AU  - Schnell, Oliver
AU  - Heiland, Dieter Henrik
AU  - Ravi, Vidhya M
TI  - Isolation and profiling of viable tumor cells from human ex vivo glioblastoma cultures through single-cell transcriptomics.
JO  - STAR Protocols
VL  - 4
IS  - 3
SN  - 2666-1667
CY  - Amsterdam
PB  - Elsevier
M1  - DKFZ-2023-01337
SP  - 102383
PY  - 2023
AB  - Single-cell RNA-sequencing (scRNA-seq) is becoming a ubiquitous method in profiling the cellular transcriptomes of both malignant and non-malignant cells from the human brain. Here, we present a protocol to isolate viable tumor cells from human ex vivo glioblastoma cultures for single-cell transcriptomic analysis. We describe steps including surgical tissue collection, sectioning, culturing, primary tumor cells inoculation, growth tracking, fluorescence-based cell sorting, and population-enriched scRNA-seq. This comprehensive methodology empowers in-depth understanding of brain tumor biology at the single-cell level. For complete details on the use and execution of this protocol, please refer to Ravi et al.1.
KW  - Cancer (Other)
KW  - Cell Biology (Other)
KW  - Cell Isolation (Other)
KW  - Flow Cytometry/Mass Cytometry (Other)
KW  - Microscopy (Other)
KW  - Model Organisms (Other)
KW  - Molecular Biology (Other)
KW  - RNAseq (Other)
KW  - Sequencing (Other)
KW  - Single Cell (Other)
KW  - Tissue Engineering (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:37393609
DO  - DOI:10.1016/j.xpro.2023.102383
UR  - https://inrepo02.dkfz.de/record/277319
ER  -