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@ARTICLE{Zhang:277319,
      author       = {J. Zhang and J. Straehle and K. Joseph and N. Neidert and
                      S. Behringer and J. Göldner and A. Vlachos and M. Prinz and
                      C. Fung and J. Beck and O. Schnell and D. H. Heiland$^*$ and
                      V. M. Ravi},
      title        = {{I}solation and profiling of viable tumor cells from human
                      ex vivo glioblastoma cultures through single-cell
                      transcriptomics.},
      journal      = {STAR Protocols},
      volume       = {4},
      number       = {3},
      issn         = {2666-1667},
      address      = {Amsterdam},
      publisher    = {Elsevier},
      reportid     = {DKFZ-2023-01337},
      pages        = {102383},
      year         = {2023},
      abstract     = {Single-cell RNA-sequencing (scRNA-seq) is becoming a
                      ubiquitous method in profiling the cellular transcriptomes
                      of both malignant and non-malignant cells from the human
                      brain. Here, we present a protocol to isolate viable tumor
                      cells from human ex vivo glioblastoma cultures for
                      single-cell transcriptomic analysis. We describe steps
                      including surgical tissue collection, sectioning, culturing,
                      primary tumor cells inoculation, growth tracking,
                      fluorescence-based cell sorting, and population-enriched
                      scRNA-seq. This comprehensive methodology empowers in-depth
                      understanding of brain tumor biology at the single-cell
                      level. For complete details on the use and execution of this
                      protocol, please refer to Ravi et al.1.},
      keywords     = {Cancer (Other) / Cell Biology (Other) / Cell Isolation
                      (Other) / Flow Cytometry/Mass Cytometry (Other) / Microscopy
                      (Other) / Model Organisms (Other) / Molecular Biology
                      (Other) / RNAseq (Other) / Sequencing (Other) / Single Cell
                      (Other) / Tissue Engineering (Other)},
      cin          = {FR01},
      ddc          = {600},
      cid          = {I:(DE-He78)FR01-20160331},
      pnm          = {899 - ohne Topic (POF4-899)},
      pid          = {G:(DE-HGF)POF4-899},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:37393609},
      doi          = {10.1016/j.xpro.2023.102383},
      url          = {https://inrepo02.dkfz.de/record/277319},
}