TY  - JOUR
AU  - Farin, Henner
AU  - Mosa, Mohammed H
AU  - Ndreshkjana, Benardina
AU  - Grebbin, Britta M
AU  - Ritter, Birgit
AU  - Menche, Constantin
AU  - Kennel, Kilian B
AU  - Ziegler, Paul K
AU  - Szabo, Lili
AU  - Bollrath, Julia
AU  - Rieder, Dietmar
AU  - Michels, Birgitta E
AU  - Kress, Alena
AU  - Bozlar, Muge
AU  - Darvishi, Tahmineh
AU  - Stier, Sara
AU  - Kur, Ivan-Maximiliano
AU  - Bankov, Katrin
AU  - Kesselring, Rebecca
AU  - Fichtner-Feigl, Stefan
AU  - Brune, Bernhard
AU  - Goetze, Thorsten O
AU  - Al-Batran, Salah-Eddin
AU  - Brandts, Christian H
AU  - Bechstein, Wolf O
AU  - Wild, Peter J
AU  - Weigert, Andreas
AU  - Muller, Susanne
AU  - Knapp, Stefan
AU  - Trajanoski, Zlatko
AU  - Greten, Florian R
TI  - Colorectal cancer organoid-stroma biobank allows subtype-specific assessment of individualized therapy responses.
JO  - Cancer discovery
VL  - 13
IS  - 10
SN  - 2159-8274
CY  - Philadelphia, Pa.
M1  - DKFZ-2023-01489
SP  - 2192-2211
PY  - 2023
N1  - 2023 Oct 5;13(10):2192-2211
AB  - In colorectal cancers (CRC) the tumor microenvironment plays a key role for prognosis and therapy efficacy. Patient-derived tumor organoids (PDTOs) show enormous potential for preclinical testing, however, cultured tumor cells lose important characteristics including the 'consensus molecular subtypes' (CMS). To better reflect the cellular heterogeneity, we established the CRC organoid-stroma biobank of matched PDTOs and cancer-associated fibroblasts (CAFs) from 30 patients. Context-specific phenotyping showed that xenotransplantation or co-culture with CAFs improves the transcriptomic fidelity and instructs subtype-specific stromal gene expression. Furthermore, functional profiling in co-culture exposed CMS4-specific therapeutic resistance to Gefitinib and SN-38 and prognostic expression signatures. Chemogenomic library screening identified patient- and therapy-dependent mechanisms of stromal resistance including MET as common target. Our results demonstrate that CRC phenotypes are encrypted in the cancer epithelium in a plastic fashion that strongly depends on the context. Consequently, CAFs are essential for faithful representation of molecular subtypes and therapy responses ex vivo.
LB  - PUB:(DE-HGF)16
C6  - pmid:37489084
DO  - DOI:10.1158/2159-8290.CD-23-0050
UR  - https://inrepo02.dkfz.de/record/277774
ER  -