Journal Article

DKFZ-2023-01489

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Colorectal cancer organoid-stroma biobank allows subtype-specific assessment of individualized therapy responses.

Farin, H.DKFZ* ; Mosa, M. H. ; Ndreshkjana, B. ; Grebbin, B. M. ; Ritter, B. ; Menche, C. ; Kennel, K. B. ; Ziegler, P. K. ; Szabo, L. ; Bollrath, J. ; Rieder, D. ; Michels, B. E. ; Kress, A. ; Bozlar, M. ; Darvishi, T. ; Stier, S. ; Kur, I.-M. ; Bankov, K. ; Kesselring, R.DKFZ* ; Fichtner-Feigl, S. ; Brune, B.DKFZ* ; Goetze, T. O. ; Al-Batran, S.-E. ; Brandts, C. H.DKFZ* ; Bechstein, W. O. ; Wild, P. J.DKFZ* ; Weigert, A.DKFZ* ; Muller, S. ; Knapp, S.DKFZ* ; Trajanoski, Z. ; Greten, F. R.DKFZ*

2023
Philadelphia, Pa.

This record in other databases:  

Please use a persistent id in citations: doi:10.1158/2159-8290.CD-23-0050

Abstract: In colorectal cancers (CRC) the tumor microenvironment plays a key role for prognosis and therapy efficacy. Patient-derived tumor organoids (PDTOs) show enormous potential for preclinical testing, however, cultured tumor cells lose important characteristics including the 'consensus molecular subtypes' (CMS). To better reflect the cellular heterogeneity, we established the CRC organoid-stroma biobank of matched PDTOs and cancer-associated fibroblasts (CAFs) from 30 patients. Context-specific phenotyping showed that xenotransplantation or co-culture with CAFs improves the transcriptomic fidelity and instructs subtype-specific stromal gene expression. Furthermore, functional profiling in co-culture exposed CMS4-specific therapeutic resistance to Gefitinib and SN-38 and prognostic expression signatures. Chemogenomic library screening identified patient- and therapy-dependent mechanisms of stromal resistance including MET as common target. Our results demonstrate that CRC phenotypes are encrypted in the cancer epithelium in a plastic fashion that strongly depends on the context. Consequently, CAFs are essential for faithful representation of molecular subtypes and therapy responses ex vivo.

Note: 2023 Oct 5;13(10):2192-2211

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Contributing Institute(s):

1. DKTK Koordinierungsstelle Frankfurt (FM01)
2. DKTK Koordinierungsstelle Freiburg (FR01)

Research Program(s):

1. 899 - ohne Topic (POF4-899) (POF4-899)

Appears in the scientific report 2023

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Database coverage:
; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Essential Science Indicators ; IF >= 25 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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