TY  - JOUR
AU  - Rubio-Lara, Juan A
AU  - Igarashi, Kyomi J
AU  - Sood, Shubhankar
AU  - Johansson, Alban
AU  - Sommerkamp, Pia
AU  - Yamashita, Masayuki
AU  - Lin, Dawn S
TI  - Expanding hematopoietic stem cell ex vivo: recent advances and technical considerations.
JO  - Experimental hematology
VL  - 125–126
IS  - September–October
SN  - 0531-5573
CY  - Amsterdam [u.a.]
PB  - Elsevier Science
M1  - DKFZ-2023-01591
SP  - 6-11
PY  - 2023
N1  - #EA:A011#LA:A010# / Volumes 125–126, September–October 2023, Pages 6-15 / Perspective
AB  - Hematopoietic stem cells (HSCs) are the most primitive cell type in the hematopoietic hierarchy, which are responsible for sustaining the lifelong production of mature blood and immune cells. Due to their superior long-term regenerative capacity, HSC therapies such as stem cell transplantation have been used in a broad range of hematological disorders. However, the rarity of this population in vivo considerably limits its clinical applications and large-scale analyses such as screening and safety studies. Therefore, ex vivo culture methods that allow long-term expansion and maintenance of functional HSCs are instrumental to overcome difficulties in studying HSC biology and improving HSC therapies. In this perspective, we discuss recent advances and technical considerations for three ex vivo HSC expansion methods including 1) polyvinyl alcohol-based HSC expansion, 2) mesenchymal stromal cell-HSC co-culture, and 3) 2D/3D hydrogel HSC culture. This review summarizes the presentations and discussions from the 2022 International Society for Experimental Hematology (ISEH) Annual Meeting New Investigator Technology Session.
LB  - PUB:(DE-HGF)16
C6  - pmid:37543237
DO  - DOI:10.1016/j.exphem.2023.07.006
UR  - https://inrepo02.dkfz.de/record/277948
ER  -