Journal Article (Review Article)

DKFZ-2023-01591

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Expanding hematopoietic stem cell ex vivo: recent advances and technical considerations.

Rubio-Lara, J. A. ; Igarashi, K. J. ; Sood, S. (First author)DKFZ* ; Johansson, A. ; Sommerkamp, P. ; Yamashita, M. ; Lin, D. S. (Last author)DKFZ*

2023
Elsevier Science Amsterdam [u.a.]

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Please use a persistent id in citations: doi:10.1016/j.exphem.2023.07.006

Abstract: Hematopoietic stem cells (HSCs) are the most primitive cell type in the hematopoietic hierarchy, which are responsible for sustaining the lifelong production of mature blood and immune cells. Due to their superior long-term regenerative capacity, HSC therapies such as stem cell transplantation have been used in a broad range of hematological disorders. However, the rarity of this population in vivo considerably limits its clinical applications and large-scale analyses such as screening and safety studies. Therefore, ex vivo culture methods that allow long-term expansion and maintenance of functional HSCs are instrumental to overcome difficulties in studying HSC biology and improving HSC therapies. In this perspective, we discuss recent advances and technical considerations for three ex vivo HSC expansion methods including 1) polyvinyl alcohol-based HSC expansion, 2) mesenchymal stromal cell-HSC co-culture, and 3) 2D/3D hydrogel HSC culture. This review summarizes the presentations and discussions from the 2022 International Society for Experimental Hematology (ISEH) Annual Meeting New Investigator Technology Session.

Note: #EA:A011#LA:A010# / Volumes 125–126, September–October 2023, Pages 6-15 / Perspective

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Contributing Institute(s):

1. A011 Stressinduzierte Aktivierung von Hämatopeotischen Stammzellen (A011)
2. A010 Stammzellen und Krebs (A010)

Research Program(s):

1. 311 - Zellbiologie und Tumorbiologie (POF4-311) (POF4-311)

Appears in the scientific report 2023

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Database coverage:
; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; Ebsco Academic Search ; Essential Science Indicators ; IF < 5 ; JCR ; Nationallizenz ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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